Prosaas/PCSK1N (Granin-Like Neuroendocrine Peptide) (ProSAAS/PCSK1N) ELISA Kit for Human

Background: PCSK1N (proprotein convertase subtilisin/kexin type 1 inhibitor), also known as SAAS or PROSAAS, is a 260 amino acid protein that is both secreted and localized to the trans-Golgi network. Expressed in pancreas and brain, PCSK1N is thought to play a role in the control of the neuroendocrine secretory pathway and may also be involved in PCSK1 inhibition.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ProSAAS/PCSK1N antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ProSAAS/PCSK1N antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ProSAAS/PCSK1N conjugated on coated antibody. After washing off unbound conjugates, HRPStreptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ProSAAS/PCSK1N in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of ProSAAS/PCSK1N elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS766146 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ProSAAS/PCSK1N (granin-like neuroendocrine peptide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ProSAAS/PCSK1N. The ELISA analytical biochemical technique of the MBS766146 kit is based on ProSAAS/PCSK1N antibody-ProSAAS/PCSK1N antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ProSAAS/PCSK1N antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ProSAAS/PCSK1N. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).