Gpsm1 (G-Protein-Signaling Modulator 1) (GPSM1) ELISA Kit for Mouse

Name: Mouse GPSM1 ELISA Kit; Gpsm1 (G-Protein-Signaling Modulator 1); MyBioSource
Brand: MyBioSource
SKU: MBS766178
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS766178

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Gpsm1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Gpsm1 antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Gpsm1 (G-protein-signaling modulator 1), ELISA Kit

Full Product Name

Mouse Gpsm1 (G-protein-signaling modulator 1)

Product Gene Name

Gpsm1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

31.25-2000pg/ml

Sensitivity

18.75pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection ofGpsm1 . No significant cross-reactivity or interference betweenGpsm1 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelGpsm1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelGpsm1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

78,114 Da

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of Gpsm1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Gpsm1 purchase

MBS766178 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gpsm1 (G-protein-signaling modulator 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Gpsm1. The ELISA analytical biochemical technique of the MBS766178 kit is based on Gpsm1 antibody-Gpsm1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gpsm1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gpsm1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Gpsm1. It may not necessarily be applicable to this product.

NCBI GI #

32189378

NCBI GeneID

67839

NCBI Accession #

NP_700459.2

UniProt Secondary Accession #

Q61366; Q8BUK4; Q8BX78; Q8R0R9; A2AIX7; A2AIX8; A2AIX9

UniProt Related Accession #

Q6IR34

GenBank Nucleotide #

NM_153410.5

NCBI Official Full Name

G-protein-signaling modulator 1 isoform 1

NCBI Official Synonym Full Names

G-protein signalling modulator 1 (AGS3-like, C. elegans)

NCBI Official Symbol

Gpsm1

NCBI Official Synonym Symbols

Ags3; AW107933; 1810037C22Rik

UniProt Protein Name

G-protein-signaling modulator 1

UniProt Synonym Protein Names

Activator of G-protein signaling 3

Protein Family

G-protein-signaling modulator

UniProt Gene Name

Gpsm1

UniProt Synonym Gene Names

Ags3

UniProt Entry Name

GPSM1_MOUSE

NCBI Protein Information

G-protein-signaling modulator 1

UniProt Comments for Gpsm1

GPSM1: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G- protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction. Belongs to the GPSM family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: cytoplasm; endoplasmic reticulum; Golgi apparatus; membrane; plasma membrane

Molecular Function: G-protein alpha-subunit binding; GDP-dissociation inhibitor activity; GTPase regulator activity; protein binding

Biological Process: cell differentiation; multicellular organismal development; nervous system development; regulation of G-protein coupled receptor protein signaling pathway

Research Articles on Gpsm1

1. We conclude that LPS priming increases AGS3 levels, which enhances lysosomal function and increases the capacity of macrophages to eliminate intracellular pathogens.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Gpsm1 elisa kit

Reference Product	PubMed Publications
Gnai3 elisa kit	>2 publications with Gpsm1 and Gnai3
Klf5 elisa kit	>1 publications with Gpsm1 and Klf5
Rgs16 elisa kit	>1 publications with Gpsm1 and Rgs16
Rgs12 elisa kit	>1 publications with Gpsm1 and Rgs12
Pard3 elisa kit	>1 publications with Gpsm1 and Pard3
Rgs14 elisa kit	>1 publications with Gpsm1 and Rgs14
Insc elisa kit	>1 publications with Gpsm1 and Insc

Pathways associated with Gpsm1 elisa kit

Products by Pathway	Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram

Diseases associated with Gpsm1 elisa kit

Disease Name	Pubmed Publications
Cocaine-Related Disorders Antibodies	>4 publications with Gpsm1 and Cocaine-Related Disorders
Disease Models, Animal Antibodies	>3 publications with Gpsm1 and Disease Models, Animal
Kidney Diseases Antibodies	>3 publications with Gpsm1 and Kidney Diseases
Inflammation Antibodies	>3 publications with Gpsm1 and Inflammation
Cardiovascular Diseases Antibodies	>2 publications with Gpsm1 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>1 publications with Gpsm1 and Breast Neoplasms
Nervous System Diseases Antibodies	>1 publications with Gpsm1 and Nervous System Diseases
Precancerous Conditions Antibodies	>1 publications with Gpsm1 and Precancerous Conditions
Necrosis Antibodies	>1 publications with Gpsm1 and Necrosis

Organs/Tissues associated with Gpsm1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>19 publications with Gpsm1 and Brain
Kidney Antibodies	>4 publications with Gpsm1 and Kidney
Bone Antibodies	>4 publications with Gpsm1 and Bone
Eye Antibodies	>3 publications with Gpsm1 and Eye
Bone Marrow Antibodies	>2 publications with Gpsm1 and Bone Marrow
Heart Antibodies	>2 publications with Gpsm1 and Heart
Connective Tissue Antibodies	>1 publications with Gpsm1 and Connective Tissue
Ovary Antibodies	>1 publications with Gpsm1 and Ovary
Thymus Antibodies	>1 publications with Gpsm1 and Thymus
Stomach Antibodies	>1 publications with Gpsm1 and Stomach