CYB5A (Cytochrome B5) (CYB5A) ELISA Kit for Mouse

Name: Mouse CYB5A ELISA Kit; CYB5A (Cytochrome B5); MyBioSource
Brand: MyBioSource
SKU: MBS766189
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS766189

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CYB5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CYB5A antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

CYB5A (Cytochrome b5), ELISA Kit

Full Product Name

Mouse CYB5A (Cytochrome b5)

Product Gene Name

CYB5A elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

15.625-1000pg/ml

Sensitivity

9.375pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection ofCYB5A . No significant cross-reactivity or interference betweenCYB5A and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelCYB5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelCYB5A were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

11,232 Da

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CYB5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYB5A purchase

MBS766189 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CYB5A (Cytochrome b5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYB5A. The ELISA analytical biochemical technique of the MBS766189 kit is based on CYB5A antibody-CYB5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYB5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYB5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CYB5A. It may not necessarily be applicable to this product.

NCBI GI #

284004897

NCBI GeneID

100328915

NCBI Accession #

NP_001164735.1

UniProt Secondary Accession #

Q28726

UniProt Related Accession #

P00169

GenBank Nucleotide #

NM_001171264.1

NCBI Official Full Name

cytochrome b5

NCBI Official Symbol

LOC100328915

NCBI Official Synonym Symbols

CYB5; CYB5A

UniProt Protein Name

Cytochrome b5

Protein Family

Cytochrome

UniProt Gene Name

CYB5A

UniProt Synonym Gene Names

CYB5

UniProt Entry Name

CYB5_RABIT

NCBI Protein Information

cytochrome b5

UniProt Comments for CYB5A

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Research Articles on CYB5A

1. the observation of weak and nonspecific interactions between CYP2B4 and cytb5 in micelles suggests that lipid bilayer structures and low curvature membrane surface are preferable for CYP2B4-cytb5 complex formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.