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G-CSF (Granulocyte Colony Stimulating Factor) (G-CSF) ELISA Kit for Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS766218

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- G-CSF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-G-CSF antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G-CSF (Granulocyte Colony Stimulating Factor), ELISA Kit
Full Product Name
Rabbit G-CSF (Granulocyte Colony Stimulating Factor)
Product Gene Name
G-CSF elisa kit
Product Synonym Names
G-CSF/CSF3/C17orf33/CSF3OS/Pluripoietin/Filgrastim/Lenograstim
Species Reactivity
Rabbit
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
46.875-3000pg/ml
Sensitivity
28.125pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of G-CSF . No significant cross-reactivity or interference between G-CSF and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GCSF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GCSF were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
18,434 Da
OMIM
138970
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of G-CSF elisa kit

Rabbit G-CSF ELISA Kit – Cat# MBS766218

Other Notes

Small volumes of G-CSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G-CSF purchase

MBS766218 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing G-CSF. The ELISA analytical biochemical technique of the MBS766218 kit is based on G-CSF antibody-G-CSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect G-CSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, G-CSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for G-CSF. It may not necessarily be applicable to this product.

NCBI GI #
743523204
NCBI GeneID
1440
NCBI Accession #
AJC19277.1
UniProt Secondary Accession #
A8MXR7
UniProt Related Accession #
P09919
NCBI Official Full Name
granulocyte-colony stimulating factor, partial
NCBI Official Synonym Full Names
colony stimulating factor 3
NCBI Official Symbol
CSF3
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Synonym Protein Names
Pluripoietin; INN: Filgrastim; INN: Lenograstim
UniProt Gene Name
CSF3
UniProt Synonym Gene Names
C17orf33; GCSF; G-CSF
UniProt Entry Name
CSF3_HUMAN
NCBI Protein Information
granulocyte colony-stimulating factor

NCBI Summary for G-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

UniProt Comments for G-CSF

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular region

Molecular Function: cytokine activity; enzyme binding; growth factor activity

Biological Process: multicellular organismal development; positive regulation of actin filament polymerization; positive regulation of myeloid cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on G-CSF

1. these data suggest that G-CSF may contribute to tumor growth and decrease the antitumor effect of radiotherapy, possibly by promoting vascularization in cancer lesions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with G-CSF elisa kit

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram

Diseases associated with G-CSF elisa kit

 Disease Name  Pubmed Publications
 Disease Susceptibility Antibodies  >7 publications with G-CSF and Disease Susceptibility
 Nervous System Diseases Antibodies  >7 publications with G-CSF and Nervous System Diseases
 Neutropenia Antibodies  >5 publications with G-CSF and Neutropenia
 Breast Neoplasms Antibodies  >3 publications with G-CSF and Breast Neoplasms
 Leukemia, Myeloid, Acute Antibodies  >3 publications with G-CSF and Leukemia, Myeloid, Acute
 Neoplasm Metastasis Antibodies  >3 publications with G-CSF and Neoplasm Metastasis
 Leukopenia Antibodies  >3 publications with G-CSF and Leukopenia
 Brain Injuries Antibodies  >2 publications with G-CSF and Brain Injuries
 Anemia Antibodies  >2 publications with G-CSF and Anemia
 Hypertrophy Antibodies  >2 publications with G-CSF and Hypertrophy

Organs/Tissues associated with G-CSF elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >10 publications with G-CSF and Lung
 Muscle Antibodies  >9 publications with G-CSF and Muscle
 Skin Antibodies  >8 publications with G-CSF and Skin
 Brain Antibodies  >4 publications with G-CSF and Brain
 Uterus Antibodies  >3 publications with G-CSF and Uterus
 Liver Antibodies  >2 publications with G-CSF and Liver
 Intestine Antibodies  >2 publications with G-CSF and Intestine
 Uncharacterized Tissue Antibodies  >1 publications with G-CSF and Uncharacterized Tissue
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