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ADHFE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS766400

MBS766400 is an antibody targeting ADHFE1. Also known as ADHFE1 Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
ADHFE1, Polyclonal Antibody
Full Product Name
ADHFE1 Rabbit Polyclonal
Product Gene Name
anti-ADHFE1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
27,353 Da
OMIM
611083
Immunogen
Alcohol dehydrogenase, iron containing, 1
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ADHFE1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-ADHFE1 antibody

Immunohistochemistry of paraffin-embedded human liver tissue slide using MBS766400 (ADHFE1 Antibody) at dilution of 1:50
Rabbit Anti-ADHFE1 Polyclonal Antibody – Cat# MBS766400

Testing Data of anti-ADHFE1 antibody

Rabbit Anti-ADHFE1 Polyclonal Antibody, Testing Data – Cat# MBS766400

Western Blot (WB) of anti-ADHFE1 antibody

mouse heart tissue were subjected to SDS PAGE followed by western blot with MBS766400 (ADHFE1 antibody) at dilution of 1:1000
Rabbit Anti-ADHFE1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS766400

Other Notes

Small volumes of anti-ADHFE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADHFE1. It may not necessarily be applicable to this product.

NCBI GI #
40352926
NCBI GeneID
137872
NCBI Accession #
AAH64634.1
UniProt Secondary Accession #
Q49A19; Q68CI7; Q6P2B6; Q96MF9; B4DTJ8
UniProt Related Accession #
Q8IWW8
NCBI Official Full Name
ADHFE1 protein
NCBI Official Synonym Full Names
alcohol dehydrogenase, iron containing 1
NCBI Official Symbol
ADHFE1
NCBI Official Synonym Symbols
HOT; ADH8; HMFT2263
UniProt Protein Name
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
UniProt Synonym Protein Names
Alcohol dehydrogenase iron-containing protein 1; ADHFe1; Fe-containing alcohol dehydrogenase
Protein Family
Hydroxyacid-oxoacid transhydrogenase
UniProt Gene Name
ADHFE1
UniProt Synonym Gene Names
HOT; ADHFe1
UniProt Entry Name
HOT_HUMAN
NCBI Protein Information
hydroxyacid-oxoacid transhydrogenase, mitochondrial

NCBI Summary for ADHFE1

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM, Mar 2008]

UniProt Comments for ADHFE1

ADHFE1: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3- hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities. Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.99.24; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 8q13.1

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: hydroxyacid-oxoacid transhydrogenase activity; metal ion binding

Biological Process: 2-oxoglutarate metabolic process; molecular hydrogen transport
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Research Articles on ADHFE1

1. These results demonstrate that the promoter hypermethylation of ADHFE1 is frequently present in CRC and alcohol induces methylation-mediated down expression of ADHFE1 and proliferation of CRC cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ADHFE1 antibody

 Products by Pathway  Pathway Diagram
 Interconversion Of 2-oxoglutarate And 2-hydroxyglutarate Pathway antibodies  Interconversion Of 2-oxoglutarate And 2-hydroxyglutarate Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway antibodies  Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway Diagram
 The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies  The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram

Diseases associated with anti-ADHFE1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with ADHFE1 and Disease Models, Animal
 Hypertrophy Antibodies  >1 publications with ADHFE1 and Hypertrophy
 Necrosis Antibodies  >1 publications with ADHFE1 and Necrosis

Organs/Tissues associated with anti-ADHFE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with ADHFE1 and Muscle
 Liver Antibodies  >2 publications with ADHFE1 and Liver
 Connective Tissue Antibodies  >1 publications with ADHFE1 and Connective Tissue
 Blood Antibodies  >1 publications with ADHFE1 and Blood
 Intestine Antibodies  >1 publications with ADHFE1 and Intestine
 Embryonic Tissue Antibodies  >1 publications with ADHFE1 and Embryonic Tissue
 Kidney Antibodies  >1 publications with ADHFE1 and Kidney
 Brain Antibodies  >1 publications with ADHFE1 and Brain
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