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ATG12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS766899

MBS766899 is an antibody targeting ATG12. Also known as ATG12 Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG12, Polyclonal Antibody
Full Product Name
ATG12 Rabbit Polyclonal
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
7,746 Da
OMIM
609608
Immunogen
ATG12 autophagy related 12 homolog (S. cerevisiae)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ATG12 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunohistochemistry (IHC) of anti-ATG12 antibody

Immunohistochemistry of paraffin-embedded human prostate cancer using MBS766899 (ATG12 antibody) at dilution of 1:50
Rabbit Anti-ATG12 Polyclonal Antibody – Cat# MBS766899

Immunofluorescence (IF) of anti-ATG12 antibody

Immunofluorescent analysis of PC3 cells using MBS766899 (ATF12 antibody) at dilution of 1:25
Rabbit Anti-ATG12 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS766899

Western Blot (WB) of anti-ATG12 antibody

human bladder tissue were subjected to SDS PAGE followed by western blot with MBS766899 (ATG12 antibody) at dilution of 1:500
Rabbit Anti-ATG12 Polyclonal Antibody, Western Blot (WB) – Cat# MBS766899

Other Notes

Small volumes of anti-ATG12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Atg12
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
UniProt Gene Name
ATG12
UniProt Synonym Gene Names
APG12; APG12L; APG12-like
UniProt Entry Name
ATG12_HUMAN
NCBI Protein Information
ubiquitin-like protein ATG12

NCBI Summary for ATG12

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ATG12

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: cytosol; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: APG8 conjugating enzyme activity; protein binding

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; innate immune response; macroautophagy; mitochondrion degradation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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