NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit for Duck

Name: Duck NQO1 ELISA Kit; NADH Dehydrogenase, Quinone 1; MyBioSource
Brand: MyBioSource
SKU: MBS076698
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS076698

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of NQO1 (hereafter termed "analyte") in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

NADH Dehydrogenase, Quinone 1 (NQO1), ELISA Kit

Full Product Name

Duck NADH Dehydrogenase, Quinone 1 ELISA Kit

Product Gene Name

NQO1 elisa kit

Species Reactivity

Duck

Assay Type

Quantitative Sandwich

Detection Range

0.5ng/ml-16ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

24,044 Da

3D Structure

ModBase 3D Structure for Q42577

Other Notes

Small volumes of NQO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NQO1 purchase

MBS076698 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NQO1. The ELISA analytical biochemical technique of the MBS076698 kit is based on NQO1 antibody-NQO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NQO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NQO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NQO1. It may not necessarily be applicable to this product.

NCBI GI #

15239782

NCBI GeneID

831049

NCBI Accession #

NP_196738.1

UniProt Primary Accession #

Q42577

UniProt Related Accession #

Q42577

GenBank Nucleotide #

NM_121215.3

NCBI Official Full Name

NADH dehydrogenase (ubiquinone) Fe-S protein 7

NCBI Official Symbol

AT5G11770

NCBI Official Synonym Symbols

T22P22.160; T22P22_160

UniProt Protein Name

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Protein Family

NAD(P)H dehydrogenase

UniProt Entry Name

NDUS7_ARATH

NCBI Protein Information

NADH dehydrogenase (ubiquinone) Fe-S protein 7

UniProt Comments for NQO1

Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

By similarity. HAMAP-Rule MF_01356

Catalytic activity: NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01356NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01356

Cofactor: Binds 1 4Fe-4S cluster

Potential.

Subunit structure: Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location: Mitochondrion Ref.6.

Sequence similarities: Belongs to the complex I 20 kDa subunit family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NQO1 elisa kit

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Oxidative Phosphorylation Pathway antibodies	Oxidative Phosphorylation Pathway Diagram
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Aerobic Respiration (alternative Oxidase Pathway) antibodies	Aerobic Respiration (alternative Oxidase Pathway) Diagram
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Aerobic Respiration (cytochrome C) Pathway antibodies	Aerobic Respiration (cytochrome C) Pathway Diagram
Aerobic Respiration (cytochrome C) Pathway antibodies	Aerobic Respiration (cytochrome C) Pathway Diagram