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CD146/MCAM Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS767661

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324). Structurally, it consists of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD146/MCAM, Monoclonal Antibody
Full Product Name
CD146/MCAM Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
CD146, MCAM, MUC18,Cell surface glycoprotein MUC18,Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule, Melanoma-associated antigen A32,Melanoma-associated antigen MUC18
Clonality
Monoclonal
Isotype
IgG1
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Purification: Protein A+G purification
Purity: > = 95% as determined by SDS-PAGE
Specificity
Human; other species are not tested. Please decide the specificity by homology
Preparation and Storage
-20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
3D Structure
ModBase 3D Structure for P43121
Immunogen
Melanoma cell adhesion molecule
Calculated MW
72kDa,Observed 120kDa
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD146/MCAM antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CD146/MCAM antibody
WB:1:500-1:5000
IHC:1:20-1:200

Immunohistochemistry (IHC) of anti-CD146/MCAM antibody

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS767661 (CD146, MCAM Antibody) at dilution of 1:20
Rabbit Anti-CD146/MCAM Monoclonal Antibody – Cat# MBS767661

Western Blot (WB) of anti-CD146/MCAM antibody

A375 cells were subjected to SDS PAGE followed by western blot with MBS767661 (CD146, MCAM antibody) at dilution of 1:1000
Rabbit Anti-CD146/MCAM Monoclonal Antibody, Western Blot (WB) – Cat# MBS767661

Other Notes

Small volumes of anti-CD146/MCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD146/MCAM purchase

MBS767661 is an antibody targeting CD146/MCAM. Also known as CD146/MCAM Mouse Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD146/MCAM. It may not necessarily be applicable to this product.

UniProt Primary Accession #

UniProt Comments for CD146/MCAM

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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