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CEP250 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS767833

MBS767833 is an antibody targeting CEP250. Also known as CEP250 Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CEP250, Polyclonal Antibody
Full Product Name
CEP250 Rabbit Polyclonal
Product Gene Name
anti-CEP250 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
274,380 Da
OMIM
609689
Immunogen
Centrosomal protein 250kDa
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CEP250 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Immunohistochemistry (IHC) of anti-CEP250 antibody

Immunohistochemistry of paraffin-embedded human placenta tissue slide using MBS767833 (CEP250 Antibody) at dilution of 1:200
Rabbit Anti-CEP250 Polyclonal Antibody – Cat# MBS767833

Immunofluorescence (IF) of anti-CEP250 antibody

Immunofluorescent analysis of (-20 degree C Ethanol) fixed HeLa cells using MBS767833 (CEP250 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L)
Rabbit Anti-CEP250 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS767833

Testing Data of anti-CEP250 antibody

Rabbit Anti-CEP250 Polyclonal Antibody, Testing Data – Cat# MBS767833

Western Blot (WB) of anti-CEP250 antibody

HeLa cells were subjected to SDS PAGE followed by western blot with MBS767833 (CEP250 antibody) at dilution of 1:500
Rabbit Anti-CEP250 Polyclonal Antibody, Western Blot (WB) – Cat# MBS767833

Other Notes

Small volumes of anti-CEP250 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CEP250. It may not necessarily be applicable to this product.

NCBI GI #
970841889
NCBI GeneID
11190
NCBI Accession #
NP_001305148.1
UniProt Secondary Accession #
O14812; O60588; Q9H450; E1P5Q3
UniProt Related Accession #
Q9BV73
GenBank Nucleotide #
NM_001318219.1
NCBI Official Full Name
centrosome-associated protein CEP250 isoform 2
NCBI Official Synonym Full Names
centrosomal protein 250
NCBI Official Symbol
CEP250
NCBI Official Synonym Symbols
CEP2; CNAP1; C-NAP1
UniProt Protein Name
Centrosome-associated protein CEP250
UniProt Synonym Protein Names
250 kDa centrosomal protein; Cep250; Centrosomal Nek2-associated protein 1; C-Nap1; Centrosomal protein 2
Protein Family
Centrosome-associated protein
UniProt Gene Name
CEP250
UniProt Synonym Gene Names
CEP2; CNAP1; Cep250; C-Nap1
UniProt Entry Name
CP250_HUMAN
NCBI Protein Information
centrosome-associated protein CEP250

NCBI Summary for CEP250

This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
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UniProt Comments for CEP250

CEP250: Probably plays an important role in centrosome cohesion during interphase. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: centriole; centrosome; cilium; cytosol; microtubule organizing center; perinuclear region of cytoplasm; protein complex

Molecular Function: protein binding; protein C-terminus binding; protein domain specific binding; protein kinase binding

Biological Process: centriole-centriole cohesion; G2/M transition of mitotic cell cycle; mitotic cell cycle; protein localization; protein localization in organelle; regulation of centriole-centriole cohesion; sensory cilium biogenesis
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Research Articles on CEP250

1. ASPP1/2 interacted with centrosome linker protein C-Nap1. Co-depletion of ASPP1 and ASPP2 inhibited re-association of C-Nap1 with centrosome at the end of mitosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CEP250 antibody

 Products by Pathway  Pathway Diagram
 AURKA Activation By TPX2 Pathway antibodies  AURKA Activation By TPX2 Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram

Diseases associated with anti-CEP250 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with CEP250 and Nervous System Diseases
 Neurologic Manifestations Antibodies  >1 publications with CEP250 and Neurologic Manifestations
 Abnormalities, Multiple Antibodies  >1 publications with CEP250 and Abnormalities, Multiple
 Birth Weight Antibodies  >1 publications with CEP250 and Birth Weight
 Weight Loss Antibodies  >1 publications with CEP250 and Weight Loss

Organs/Tissues associated with anti-CEP250 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with CEP250 and Testis
 Blood Antibodies  >1 publications with CEP250 and Blood
 Eye Antibodies  >1 publications with CEP250 and Eye
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