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CMBL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8105304

Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol-lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of… More details

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Product Overview

Online Datasheet in PDF
Product Name
CMBL, Polyclonal Antibody
Full Product Name
Anti-CMBL Antibody, Rabbit Polyclonal
Product Gene Name
anti-CMBL antibody
Product Synonym Names
CMBL Antibody, Rabbit PAb, Antigen Affinity Purified; carboxymethylenebutenolidase homolog (Pseudomonas); Anti-JS-1 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human CMBL
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Molecular Weight
28,048 Da
OMIM
613379
3D Structure
ModBase 3D Structure for Q96DG6
Immunogen
Recombinant Human CMBL Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CMBL (rh CMBL). CMBL specific IgG was purified by Human CMBL affinity chromatography.
Applications Tested/Suitable for anti-CMBL antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes for anti-CMBL antibody
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate

Western Blot (WB) of anti-CMBL antibody

Anti-CMBL rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate

Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.
Performed under reducing conditions.

Predicted band size:28 kDa
Observed band size:28 kDa
Rabbit Anti-CMBL Polyclonal Antibody – Cat# MBS8105304

Immunoprecipitation (IP) of anti-CMBL antibody

CMBL was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg Jurkat Whole Cell Lysate
Lane C:0.5 mg HepG2 Whole Cell Lysate

4 uL anti-CMBL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.

Primary antibody:
Anti-CMBL rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.
Performed under reducing conditions.

Predicted band size: 28 kDa
Observed band size: 28 kDa
Rabbit Anti-CMBL Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8105304

Product References and Citations for anti-CMBL antibody

Ishizuka T, et al. (2010) Human Carboxymethylenebutenolidase as a Bioactivating Hydrolase of Olmesartan Medoxomil in Liver and Intestine. The Journal of Biological Chemistry. 285: 11892-902.Schmidt E, et al. (1980) Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem J. 192 (1): 339-47.

Other Notes

Small volumes of anti-CMBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CMBL purchase

MBS8105304 is an antibody targeting CMBL. Also known as Anti-CMBL Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CMBL. It may not necessarily be applicable to this product.

NCBI GI #
20270371
NCBI GeneID
134147
NCBI Accession #
NP_620164.1
UniProt Primary Accession #
Q96DG6
UniProt Secondary Accession #
Q8TED6; D3DTC7
UniProt Related Accession #
Q96DG6
GenBank Nucleotide #
NM_138809.3
NCBI Official Full Name
carboxymethylenebutenolidase homolog
NCBI Official Synonym Full Names
carboxymethylenebutenolidase homolog (Pseudomonas)
NCBI Official Symbol
CMBL
NCBI Official Synonym Symbols
JS-1
UniProt Protein Name
Carboxymethylenebutenolidase homolog
Protein Family
Carboxymethylenebutenolidase
UniProt Gene Name
CMBL
UniProt Entry Name
CMBL_HUMAN
NCBI Protein Information
carboxymethylenebutenolidase homolog; carboxymethylenebutenolidase-like (Pseudomonas)

NCBI Summary for CMBL

CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]
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UniProt Comments for CMBL

CMBL: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. Belongs to the dienelactone hydrolase family.

Protein type: EC 3.1.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytosol

Molecular Function: hydrolase activity

Biological Process: metabolic process
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Research Articles on CMBL

1. By comparing the enzyme kinetics and chemical inhibition properties between the recombinant protein and tissue preparations, CMBL was shown to be the primary olmesartan medoxomil bioactivating enzyme in the liver and intestine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CMBL antibody

 Reference Product  PubMed Publications
 AGTR1 antibody  >1 publications with CMBL and AGTR1

Pathways associated with anti-CMBL antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-CMBL antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >24 publications with CMBL and Necrosis
 Nervous System Diseases Antibodies  >22 publications with CMBL and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >20 publications with CMBL and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >16 publications with CMBL and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >15 publications with CMBL and Disease Models, Animal
 Inflammation Antibodies  >14 publications with CMBL and Inflammation
 Heart Diseases Antibodies  >14 publications with CMBL and Heart Diseases
 Breast Neoplasms Antibodies  >12 publications with CMBL and Breast Neoplasms
 Lung Neoplasms Antibodies  >10 publications with CMBL and Lung Neoplasms
 Anemia Antibodies  >7 publications with CMBL and Anemia

Organs/Tissues associated with anti-CMBL antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >49 publications with CMBL and Muscle
 Brain Antibodies  >42 publications with CMBL and Brain
 Liver Antibodies  >36 publications with CMBL and Liver
 Bone Antibodies  >30 publications with CMBL and Bone
 Skin Antibodies  >25 publications with CMBL and Skin
 Heart Antibodies  >24 publications with CMBL and Heart
 Vascular Antibodies  >24 publications with CMBL and Vascular
 Lung Antibodies  >17 publications with CMBL and Lung
 Embryonic Tissue Antibodies  >14 publications with CMBL and Embryonic Tissue
 Prostate Antibodies  >12 publications with CMBL and Prostate
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