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CPSF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS768170

MBS768170 is an antibody targeting CPSF3. Also known as CPSF3 Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CPSF3, Polyclonal Antibody
Full Product Name
CPSF3 Rabbit Polyclonal
Product Gene Name
anti-CPSF3 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
77,486 Da
OMIM
606029
Immunogen
Cleavage and polyadenylation specific factor 3, 73kDa
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CPSF3 antibody
ELISA (EIA), Western Blot (WB)

Western Blot (WB) of anti-CPSF3 antibody

HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS768170 (CPSF3 Antibody) at dilution of 1:1000
Rabbit Anti-CPSF3 Polyclonal Antibody – Cat# MBS768170

Other Notes

Small volumes of anti-CPSF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CPSF3. It may not necessarily be applicable to this product.

NCBI GI #
1012443713
NCBI GeneID
51692
NCBI Accession #
NP_001308764.1
UniProt Secondary Accession #
O14769; Q53RS2; Q96F36
UniProt Related Accession #
Q9UKF6
GenBank Nucleotide #
NM_001321835.1
NCBI Official Full Name
cleavage and polyadenylation specificity factor subunit 3 isoform c
NCBI Official Synonym Full Names
cleavage and polyadenylation specific factor 3
NCBI Official Symbol
CPSF3
NCBI Official Synonym Symbols
CPSF73; CPSF-73
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 3
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73
Protein Family
Cleavage and polyadenylation specificity factor
UniProt Gene Name
CPSF3
UniProt Synonym Gene Names
CPSF73; CPSF 73 kDa subunit
UniProt Entry Name
CPSF3_HUMAN
NCBI Protein Information
cleavage and polyadenylation specificity factor subunit 3

NCBI Summary for CPSF3

This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]
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UniProt Comments for CPSF3

CPSF3: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.

Protein type: RNA-binding; EC 3.1.27.-; RNA splicing; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm; ribonucleoprotein complex

Molecular Function: 5'-3' exonuclease activity; endoribonuclease activity; metal ion binding; protein binding; RNA binding

Biological Process: histone mRNA 3'-end processing; mRNA 3'-end processing; mRNA cleavage; mRNA export from nucleus; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription
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Research Articles on CPSF3

1. the molecular mechanism that recruits the CPSF73 endonuclease to histone pre-mRNAs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CPSF3 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram
 Transport Of Mature MRNA Derived From An Intronless Transcript Pathway antibodies  Transport Of Mature MRNA Derived From An Intronless Transcript Pathway Diagram
 Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway antibodies  Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway Diagram

Diseases associated with anti-CPSF3 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with CPSF3 and Kidney Diseases

Organs/Tissues associated with anti-CPSF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with CPSF3 and Muscle
 Prostate Antibodies  >1 publications with CPSF3 and Prostate
 Kidney Antibodies  >1 publications with CPSF3 and Kidney
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