CUL1 Antibody – Rabbit Polyclonal

CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: cullin-RING ubiquitin ligase complex; cytosol; nucleoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cell cycle arrest; cell proliferation; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of cell proliferation; organ morphogenesis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; viral reproduction; Wnt receptor signaling pathway