• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DBI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9133339
SPECIAL PROMOTION: Get FREE $5 Amazon gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DBI, Polyclonal Antibody
Full Product Name
DBI Polyclonal Antibody
Product Gene Name
anti-DBI antibody
Product Synonym Names
ACBD1; ACBP; CCK-RP; EP
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 12 kDa
Calculated MW: 10-12 kDa
OMIM
M14200 mRNA
3D Structure
ModBase 3D Structure for P07108
Immunogen
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cross/Species Reactivity
Human, Mouse, Rat

Product Categories/Family for anti-DBI antibody

Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DBI antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-DBI antibody
WB: 1:500 - 1:1000
IF/ICC: 1:50 - 1:200
IP: 0.5 ug- 4 ug antibody for 200 ug- 400 ug extracts of whole cells
ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.

Western Blot (WB) of anti-DBI antibody

Western blot analysis of various lysates using DBI Rabbit pAb (MBS9133339) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (Please Inquire).
Exposure time: 1s.
Rabbit Anti-DBI Polyclonal Antibody – Cat# MBS9133339

Western Blot (WB) of anti-DBI antibody

Western blot analysis of various lysates using DBI Rabbit pAb (MBS9133339) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (Please Inquire).
Exposure time: 180s.
Rabbit Anti-DBI Polyclonal Antibody, Western Blot (WB) – Cat# MBS9133339

Immunofluorescence (IF) of anti-DBI antibody

Immunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (MBS9133339) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-DBI Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133339

Immunofluorescence (IF) of anti-DBI antibody

Immunofluorescence analysis of U2OS cells using DBI Rabbit pAb (MBS9133339) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-DBI Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133339

Immunoprecipitation (IP) of anti-DBI antibody

Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg DBI antibody (MBS9133339). Western blot was performed from the immunoprecipitate using DBI antibody (MBS9133339) at a dilution of 1:1000.
Rabbit Anti-DBI Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS9133339

Immunofluorescence (IF) of anti-DBI antibody

Immunofluorescence analysis of A-549 cells using DBI Rabbit pAb (MBS9133339) at dilution of 1:100 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-DBI Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133339

Immunofluorescence (IF) of anti-DBI antibody

Immunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (MBS9133339) at dilution of 1:100 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-DBI Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133339

Other Notes

Small volumes of anti-DBI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBI purchase

MBS9133339 is an antibody targeting DBI. Also known as DBI Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DBI. It may not necessarily be applicable to this product.

NCBI GI #
120433590
NCBI GeneID
1622
NCBI Accession #
NP_001073331.1
UniProt Primary Accession #
P07108
UniProt Secondary Accession #
P08869; Q4VWZ6; Q53SQ7; Q6IB48; Q9UCI8; B8ZWD2; B8ZWD6; B8ZWD7
UniProt Related Accession #
P07108
GenBank Nucleotide #
NM_001079862.2
NCBI Official Full Name
acyl-CoA-binding protein isoform 3
NCBI Official Synonym Full Names
diazepam binding inhibitor, acyl-CoA binding protein
NCBI Official Symbol
DBI
NCBI Official Synonym Symbols
EP; ACBP; ACBD1; CCK-RP
UniProt Protein Name
Acyl-CoA-binding protein
UniProt Synonym Protein Names
Diazepam-binding inhibitor; DBI; Endozepine; EP
Protein Family
Acyl-CoA-binding protein
UniProt Gene Name
DBI
UniProt Synonym Gene Names
ACBP; DBI; EP
NCBI Protein Information
acyl-CoA-binding protein

UniProt Comments for DBI

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Show more

Research Articles on DBI

1. ACBP increases the activity of ceramide synthase 2 (CerS2) by more than 2-fold and CerS3 activity by 7-fold. ACBP binds very-long-chain acyl-CoA esters, which is required for its ability to stimulate CerS activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DBI antibody

 Reference Product  PubMed Publications
 TSPO antibody  >7 publications with DBI and TSPO
 CCK antibody  >5 publications with DBI and CCK
 STAR antibody  >2 publications with DBI and STAR
 PPARG antibody  >2 publications with DBI and PPARG

Pathways associated with anti-DBI antibody

 Products by Pathway  Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 SREBP Signalling Pathway antibodies  SREBP Signalling Pathway Diagram

Diseases associated with anti-DBI antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >78 publications with DBI and Nervous System Diseases
 Disease Models, Animal Antibodies  >30 publications with DBI and Disease Models, Animal
 Hemorrhage Antibodies  >15 publications with DBI and Hemorrhage
 Liver Diseases Antibodies  >14 publications with DBI and Liver Diseases
 Necrosis Antibodies  >8 publications with DBI and Necrosis
 Neoplasms, Experimental Antibodies  >8 publications with DBI and Neoplasms, Experimental
 Hepatic Encephalopathy Antibodies  >7 publications with DBI and Hepatic Encephalopathy
 Edema Antibodies  >7 publications with DBI and Edema
 Kidney Diseases Antibodies  >7 publications with DBI and Kidney Diseases
 Carcinoma Antibodies  >7 publications with DBI and Carcinoma

Organs/Tissues associated with anti-DBI antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >205 publications with DBI and Brain
 Blood Antibodies  >98 publications with DBI and Blood
 Nerve Antibodies  >35 publications with DBI and Nerve
 Liver Antibodies  >34 publications with DBI and Liver
 Vascular Antibodies  >31 publications with DBI and Vascular
 Adrenal Gland Antibodies  >23 publications with DBI and Adrenal Gland
 Kidney Antibodies  >19 publications with DBI and Kidney
 Testis Antibodies  >19 publications with DBI and Testis
 Heart Antibodies  >15 publications with DBI and Heart
 Muscle Antibodies  >15 publications with DBI and Muscle
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000