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DCI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS768496

MBS768496 is an antibody targeting DCI. Also known as DCI Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DCI, Polyclonal Antibody
Full Product Name
DCI Rabbit Polyclonal
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
30,896 Da
OMIM
600305
Immunogen
Dodecenoyl-Coenzyme A delta isomerase (3, 2 trans-enoyl-Coenzyme A isomerase)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-DCI antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)

Immunohistochemistry (IHC) of anti-DCI antibody

Immunohistochemistry of paraffin-embedded human prostate cancer using MBS768496 (DCI antibody) at dilution of 1:50
Rabbit Anti-DCI Polyclonal Antibody – Cat# MBS768496

Immunofluorescence (IF) of anti-DCI antibody

Immunofluorescent analysis of Hela cells using MBS768496 (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Rabbit Anti-DCI Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS768496

Western Blot (WB) of anti-DCI antibody

HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS768496 (DCI Antibody) at dilution of 1:600
Rabbit Anti-DCI Polyclonal Antibody, Western Blot (WB) – Cat# MBS768496

Other Notes

Small volumes of anti-DCI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DCI protein
NCBI Official Synonym Full Names
enoyl-CoA delta isomerase 1
NCBI Official Symbol
ECI1
NCBI Official Synonym Symbols
DCI
UniProt Protein Name
Enoyl-CoA delta isomerase 1, mitochondrial
UniProt Synonym Protein Names
3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase
UniProt Gene Name
ECI1
UniProt Synonym Gene Names
DCI; D3,D2-enoyl-CoA isomerase
UniProt Entry Name
ECI1_HUMAN
NCBI Protein Information
enoyl-CoA delta isomerase 1, mitochondrial

NCBI Summary for DCI

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
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UniProt Comments for DCI

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; Lipid Metabolism - fatty acid; Mitochondrial; EC 5.3.3.8

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: dodecenoyl-CoA delta-isomerase activity; identical protein binding; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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