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Ligase I (DNA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS768663

MBS768663 is an antibody targeting DNA. Also known as DNA Ligase I Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DNA Ligase I, Polyclonal Antibody
Full Product Name
DNA Ligase I Rabbit Polyclonal
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
102,482 Da
Immunogen
Ligase I, DNA, ATP-dependent
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-DNA antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Immunohistochemistry (IHC) of anti-DNA antibody

Immunohistochemistry of paraffin-embedded human spleen using MBS768663 (LIG1 antibody) at dilution of 1:100
Rabbit Anti-DNA Polyclonal Antibody – Cat# MBS768663

Testing Data of anti-DNA antibody

Rabbit Anti-DNA Polyclonal Antibody, Testing Data – Cat# MBS768663

Western Blot (WB) of anti-DNA antibody

HepG2 cells were subjected to SDS PAGE followed by western blot with MBS768663 (LIG1 antibody) at dilution of 1:500
Rabbit Anti-DNA Polyclonal Antibody, Western Blot (WB) – Cat# MBS768663

Other Notes

Small volumes of anti-DNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DNA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA ligase 1
NCBI Official Synonym Full Names
DNA ligase 1
NCBI Official Symbol
Lig1
UniProt Protein Name
DNA ligase 1
UniProt Synonym Protein Names
DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
Protein Family
UniProt Gene Name
Lig1
UniProt Entry Name
DNLI1_RAT
NCBI Protein Information
DNA ligase 1

NCBI Summary for DNA

catalyzes the joining of blunt ended DNA [RGD, Feb 2006]

UniProt Comments for DNA

LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Protein type: DNA repair, damage; EC 6.5.1.1; Ligase

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleoplasm; nucleus

Molecular Function: ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; metal ion binding

Biological Process: base-excision repair; cell division; DNA ligation; DNA ligation during DNA repair; DNA recombination; DNA repair; DNA replication; double-strand break repair via nonhomologous end joining; response to hydrogen peroxide; V(D)J recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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