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EIF4A2 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178153

Description: Rabbit IgG polyclonal antibody for Eukaryotic initiation factor 4A-II(EIF4A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is… More details

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Product Overview

Online Datasheet in PDF
Product Name
eIF4A2, Polyclonal Antibody
Full Product Name
Anti-eIF4A2 Antibody
Product Gene Name
anti-EIF4A2 antibody
Product Synonym Names
Eukaryotic initiation factor 4A-II; ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; eIF 4A II; eIF-4A-II; EIF4A; eIF4A II; eIF4A-II; EIF4A2; EIF4F; Eukaryotic initiation factor 4A II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; Eukaryotic translation initiation factor 4A2; IF4A2_HUMAN; N-terminally processed; eukaryotic translation initiation factor 4A2
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
46,489 Da
OMIM
601102
3D Structure
ModBase 3D Structure for Q14240
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2 (5-31aa SADYNREHGGPEGMDPDGVIESNWNEI), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EIF4A2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-EIF4A2 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-EIF4A2 antibody

Anti- eIF4A2 Picoband antibody, MBS178153, Western blotting
All lanes: Anti eIF4A2 (MBS178153) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SGC Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Anti-EIF4A2 Polyclonal Antibody – Cat# MBS178153

Immunohistochemistry (IHC) of anti-EIF4A2 antibody

Anti- eIF4A2 Picoband antibody, MBS178153, IHC(P)
IHC(P): Mouse Spleen Tissue
Anti-EIF4A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178153

Immunohistochemistry (IHC) of anti-EIF4A2 antibody

Anti- eIF4A2 Picoband antibody, MBS178153, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-EIF4A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178153

Immunohistochemistry (IHC) of anti-EIF4A2 antibody

Anti- eIF4A2 Picoband antibody, MBS178153, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-EIF4A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178153

Product References and Citations for anti-EIF4A2 antibody

1. "Entrez Gene: EIF4A2 eukaryotic translation initiation factor 4A, isoform 2". 2. Meijer, H. A., Kong, Y. W., Lu, W. T., Wilczynska, A., Spriggs, R. V., Robinson, S. W., Godfrey, J. D., Willis, A. E., Bushell, M. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340: 82-85, 2013. 3. Sudo K, Takahashi E, Nakamura Y (Jan 1996). "Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2". Cytogenet Cell Genet 71 (4): 385-8.

Other Notes

Small volumes of anti-EIF4A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4A2 purchase

MBS178153 is an antibody targeting EIF4A2. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4A2. It may not necessarily be applicable to this product.

NCBI GI #
83700235
NCBI GeneID
1974
NCBI Accession #
NP_001958.2
UniProt Primary Accession #
Q14240
UniProt Secondary Accession #
Q53XJ6; Q96B90; Q96EA8; D3DNU9
UniProt Related Accession #
Q14240
GenBank Nucleotide #
NM_001967.3
NCBI Official Full Name
eukaryotic initiation factor 4A-II
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A2
NCBI Official Symbol
EIF4A2
NCBI Official Synonym Symbols
DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
UniProt Protein Name
Eukaryotic initiation factor 4A-II
UniProt Synonym Protein Names
ATP-dependent RNA helicase eIF4A-2
Protein Family
Eukaryotic initiation factor
UniProt Gene Name
EIF4A2
UniProt Synonym Gene Names
DDX2B; EIF4F; eIF-4A-II; eIF4A-II
UniProt Entry Name
IF4A2_HUMAN
NCBI Protein Information
eukaryotic initiation factor 4A-II

UniProt Comments for EIF4A2

EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation initiation; Translation; EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; perinuclear region of cytoplasm

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; ATPase activity; helicase activity; protein binding; translation initiation factor activity

Biological Process: poly(A) tail shortening; regulation of translational initiation; RNA secondary structure unwinding; translational initiation; viral reproduction
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Research Articles on EIF4A2

1. expression is an independent prognostic factor for patients with non-small-cell lung cancer for both overall survival and disease-free survival

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4A2 antibody

 Reference Product  PubMed Publications
 EIF4A1 antibody  >7 publications with EIF4A2 and EIF4A1
 EIF4E antibody  >1 publications with EIF4A2 and EIF4E
 EIF4G1 antibody  >1 publications with EIF4A2 and EIF4G1

Pathways associated with anti-EIF4A2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram

Diseases associated with anti-EIF4A2 antibody

 Disease Name  Pubmed Publications
 Memory Disorders Antibodies  >1 publications with EIF4A2 and Memory Disorders
 Lung Diseases Antibodies  >1 publications with EIF4A2 and Lung Diseases
 Carcinoma, Squamous Cell Antibodies  >1 publications with EIF4A2 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >1 publications with EIF4A2 and Lung Neoplasms
 Fatty Liver Antibodies  >1 publications with EIF4A2 and Fatty Liver
 Fibrosis Antibodies  >1 publications with EIF4A2 and Fibrosis
 Liver Diseases Antibodies  >1 publications with EIF4A2 and Liver Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with EIF4A2 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-EIF4A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with EIF4A2 and Liver
 Muscle Antibodies  >3 publications with EIF4A2 and Muscle
 Blood Antibodies  >3 publications with EIF4A2 and Blood
 Brain Antibodies  >3 publications with EIF4A2 and Brain
 Ovary Antibodies  >1 publications with EIF4A2 and Ovary
 Pancreas Antibodies  >1 publications with EIF4A2 and Pancreas
 Lung Antibodies  >1 publications with EIF4A2 and Lung
 Embryonic Tissue Antibodies  >1 publications with EIF4A2 and Embryonic Tissue
 Kidney Antibodies  >1 publications with EIF4A2 and Kidney
 Nerve Antibodies  >1 publications with EIF4A2 and Nerve
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