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EIF5B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS768962

MBS768962 is an antibody targeting EIF5B. Also known as EIF5B Rabbit Polyclonal. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
EIF5B, Polyclonal Antibody
Full Product Name
EIF5B Rabbit Polyclonal
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Molecular Weight
138,827 Da
OMIM
606086
Immunogen
Eukaryotic translation initiation factor 5B
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EIF5B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunohistochemistry (IHC) of anti-EIF5B antibody

Immunohistochemistry of paraffin-embedded human gliomas using MBS768962 (EIF5B antibody) at dilution of 1:100
Rabbit Anti-EIF5B Polyclonal Antibody – Cat# MBS768962

Western Blot (WB) of anti-EIF5B antibody

mouse brain tissue were subjected to SDS PAGE followed by western blot with MBS768962 (EIF5B antibody) at dilution of 1:500
Rabbit Anti-EIF5B Polyclonal Antibody, Western Blot (WB) – Cat# MBS768962

Other Notes

Small volumes of anti-EIF5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF5B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
EIF5B protein, partial
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5B
NCBI Official Symbol
EIF5B
NCBI Official Synonym Symbols
IF2
UniProt Protein Name
Eukaryotic translation initiation factor 5B
UniProt Synonym Protein Names
Translation initiation factor IF-2
UniProt Gene Name
EIF5B
UniProt Synonym Gene Names
IF2; KIAA0741; eIF-5B
UniProt Entry Name
IF2P_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 5B

NCBI Summary for EIF5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
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UniProt Comments for EIF5B

EIF5B: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. Belongs to the IF-2 family.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: GTP binding; GTPase activity; metal ion binding; protein binding; translation initiation factor activity

Biological Process: regulation of translational initiation; translational initiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-EIF5B antibody

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