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GORASP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9609853

Function: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of… More details

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Product Overview

Online Datasheet in PDF
Product Name
GORASP1, Polyclonal Antibody
Full Product Name
GORASP1 Antibody
Product Gene Name
anti-GORASP1 antibody
Product Synonym Names
FLJ23443; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; Golgi reassembly and stacking protein 65 kDa; Golgi reassembly and stacking protein, 65-kD; Golgi reassembly stacking protein 1 65kDa; Golgi reassembly stacking protein 1; Golgi reassembly stacking protein of 65 kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH 5; GOLPH5; Gorasp 1; GORASP1; GORS1_HUMAN; GRASP 65; GRASP65; MGC118894; MGC118897; P65
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
GORASP1 antibody detects endogenous levels of total GORASP1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
GORASP1 peptide (MBS9628557) is used for blocking the activity of GORASP1 antibody (MBS9609853)
Molecular Weight
Observed: 46 kDa
Predicted: 47 kDa
3D Structure
ModBase 3D Structure for Q9BQQ3
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human GORASP1
Subcellular Location
Golgi Apparatus > Cis-Golgi Network Membrane. Undergoes rapid exchange with the cytosol.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GORASP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-GORASP1 antibody
WB: 1:1000-3000
IHC: 1:200

Western Blot (WB) of anti-GORASP1 antibody

Western blot analysis GORASP1 using Jurkat whole cell lysates
Rabbit Anti-GORASP1 Polyclonal Antibody – Cat# MBS9609853

Immunohistochemistry (IHC) of anti-GORASP1 antibody

MBS9609853 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-GORASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9609853

Western Blot (WB) of anti-GORASP1 antibody

Western blot analysis of extracts from mouse brain, using GORASP1 Antibody.
Rabbit Anti-GORASP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9609853

Other Notes

Small volumes of anti-GORASP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GORASP1 purchase

MBS9609853 is an antibody targeting GORASP1. Also known as GORASP1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GORASP1. It may not necessarily be applicable to this product.

NCBI GI #
525313685
NCBI GeneID
64689
NCBI Accession #
NP_001265718.1
UniProt Primary Accession #
Q9BQQ3
UniProt Secondary Accession #
Q3SYG7; Q8N272; Q96H42; B3KWC8
UniProt Related Accession #
Q9BQQ3
GenBank Nucleotide #
NM_001278789.1
NCBI Official Full Name
Golgi reassembly-stacking protein 1 isoform 2
NCBI Official Synonym Full Names
golgi reassembly stacking protein 1
NCBI Official Symbol
GORASP1
NCBI Official Synonym Symbols
P65; GOLPH5; GRASP65
UniProt Protein Name
Golgi reassembly-stacking protein 1
UniProt Synonym Protein Names
Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; GOLPH5; Golgi reassembly-stacking protein of 65 kDa; GRASP65
Protein Family
Golgi reassembly-stacking protein
UniProt Gene Name
GORASP1
UniProt Synonym Gene Names
GOLPH5; GRASP65; GOLPH5; GRASP65
NCBI Protein Information
Golgi reassembly-stacking protein 1

NCBI Summary for GORASP1

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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UniProt Comments for GORASP1

Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:26363069). Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344).
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Research Articles on GORASP1

1. In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GORASP1 antibody

 Reference Product  PubMed Publications
 CASP3 antibody  >2 publications with GORASP1 and CASP3
 PLK1 antibody  >1 publications with GORASP1 and PLK1
 CDK1 antibody  >1 publications with GORASP1 and CDK1

Pathways associated with anti-GORASP1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 Golgi Cisternae Pericentriolar Stack Reorganization Pathway antibodies  Golgi Cisternae Pericentriolar Stack Reorganization Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic M-M/G1 Phases Pathway antibodies  Mitotic M-M/G1 Phases Pathway Diagram
 Mitotic Prophase Pathway antibodies  Mitotic Prophase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram

Diseases associated with anti-GORASP1 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with GORASP1 and Kidney Diseases
 Lung Neoplasms Antibodies  >1 publications with GORASP1 and Lung Neoplasms

Organs/Tissues associated with anti-GORASP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with GORASP1 and Liver
 Kidney Antibodies  >3 publications with GORASP1 and Kidney
 Pancreas Antibodies  >1 publications with GORASP1 and Pancreas
 Skin Antibodies  >1 publications with GORASP1 and Skin
 Placenta Antibodies  >1 publications with GORASP1 and Placenta
 Lung Antibodies  >1 publications with GORASP1 and Lung
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