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VIPR1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9606624

MBS9606624 is an antibody targeting VIPR1. Also known as VIPR1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VIPR1, Polyclonal Antibody
Full Product Name
VIPR1 Antibody
Product Gene Name
Product Synonym Names
FLJ41949; HVR 1; HVR1; PACAP R 2; PACAP type II receptor; PACAP-R-2; PACAP-R2; Pituitary adenylate cyclase activating polypeptide receptor type II; Pituitary adenylate cyclase activating polypeptide type II receptor; Pituitary adenylate cyclase-activating polypeptide type II receptor; RCD1; RDC 1; RDC1; V1RG; VAPC 1; VAPC1; Vasoactive intestinal peptide receptor 1; Vasoactive intestinal polypeptide receptor 1; Vasoactive intestinal polypeptide receptor 1 precursor; VIP and PACAP receptor 1; VIP R 1; VIP receptor type I; VIP-R-1; VIPR 1; VIPR; VIPR1; VIPR1_HUMAN; VIRG; VPAC 1; VPAC1; VPCAP1R
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
~1mg/ml (lot specific)
Specificity
VIPR1 antibody detects endogenous levels of total VIPR1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Molecular Weight
52KD
3D Structure
ModBase 3D Structure for P32241
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human VIP Receptor 1, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-VIPR1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-VIPR1 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Immunofluorescence (IF) of anti-VIPR1 antibody

MBS9606624 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-VIPR1 Polyclonal Antibody – Cat# MBS9606624

Western Blot (WB) of anti-VIPR1 antibody

Western blot analysis of extracts from HT-29 cells using VIPR1 antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-VIPR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606624

Other Notes

Small volumes of anti-VIPR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VIPR1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vasoactive intestinal polypeptide receptor 1 isoform 2
NCBI Official Synonym Full Names
vasoactive intestinal peptide receptor 1
NCBI Official Symbol
VIPR1
NCBI Official Synonym Symbols
II; HVR1; RDC1; V1RG; VIPR; VIRG; VAPC1; VPAC1; VPAC1R; VIP-R-1; VPCAP1R; PACAP-R2; PACAP-R-2
UniProt Protein Name
Vasoactive intestinal polypeptide receptor 1
UniProt Synonym Protein Names
Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1
UniProt Gene Name
VIPR1
UniProt Synonym Gene Names
VIP-R-1; PACAP type II receptor; PACAP-R-2; PACAP-R2
NCBI Protein Information
vasoactive intestinal polypeptide receptor 1

NCBI Summary for VIPR1

This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for VIPR1

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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