Mouse Anti-SHANK2 Monoclonal Antibody – Cat# MBS800052

Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

SHANK2 Antibody – Mouse Monoclonal

Name: SHANK2 Antibody; Monoclonal; Mouse; Mouse/Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS800052
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800052

Background Info: Detects ~160kDa. No cross-reactivity against Shank1 or Shank3.Scientific Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Shank2, Monoclonal Antibody

Full Product Name

SHANK2 Antibody: RPE

Product Gene Name

anti-Shank2 antibody

Product Synonym Names

Shank2; PROSAP1; cortactin binding protein 1; KIAA1022; AUTS17; CortBP1; Proline-rich synapse-associated protein 1; SH3 and multiple ankyrin repeat domains protein 2; Cortactin-binding protein 1; proline-rich synapse associated protein 1; SPANK-3

Clonality

Monoclonal

Isotype

IgG1

Clone Number

S23b-6

Host

Mouse

Species Reactivity

Mouse (weak), Human, Rat

Form/Format

Protein G Purified

Concentration

1mg/mL (lot specific)

Preparation and Storage

-20 degree C

Molecular Weight

199,855 Da

3D Structure

ModBase 3D Structure for Q9QX74

Conjugate

RPE

Storage Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Shank2 antibody

Neuroscience; Scaffolds

Applications Tested/Suitable for anti-Shank2 antibody

Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Application Notes for anti-Shank2 antibody

1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Product References and Citations for anti-Shank2 antibody

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 4. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 5. Gong Y., et al. (2009) Brain Res. 1292: 191-198.

Other Notes

Small volumes of anti-Shank2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Shank2 purchase

MBS800052 is an antibody targeting Shank2. Also known as SHANK2 Antibody: RPE. Reactive in Mouse (weak), Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Shank2. It may not necessarily be applicable to this product.

NCBI GI #

41235797

NCBI GeneID

171093

NCBI Accession #

NP_958738.1

UniProt Primary Accession #

Q9QX74

UniProt Secondary Accession #

O70470; Q6WB19; Q9QX93; Q9QZZ9; Q9WUV9; Q9WUW0; Q9WV46

UniProt Related Accession #

Q9QX74

GenBank Nucleotide #

NM_201350.1

NCBI Official Full Name

SH3 and multiple ankyrin repeat domains protein 2 isoform a

NCBI Official Synonym Full Names

SH3 and multiple ankyrin repeat domains 2

NCBI Official Symbol

Shank2

NCBI Official Synonym Symbols

CortBP1; ProSAP1; Spank-3

UniProt Protein Name

SH3 and multiple ankyrin repeat domains protein 2

UniProt Synonym Protein Names

Cortactin-binding protein 1; CortBP1; GKAP/SAPAP-interacting protein; Proline-rich synapse-associated protein 1; ProSAP1; SPANK-3

Protein Family

SH3 and multiple ankyrin repeat domains protein

UniProt Gene Name

Shank2

UniProt Synonym Gene Names

Cortbp1; Shank2; CortBP1; ProSAP1

UniProt Entry Name

SHAN2_RAT

NCBI Protein Information

SH3 and multiple ankyrin repeat domains protein 2; SH3/ankyrin domain gene 2; cortactin-binding protein 1; GKAP/SAPAP-interacting protein; proline rich synapse associated protein 1; proline-rich synapse-associated protein 1

NCBI Summary for Shank2

binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

UniProt Comments for Shank2

SHANK2: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Defects in SHANK2 are a cause of susceptibility to autism type 17 (AUTS17). Autism is a complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. Belongs to the SHANK family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: neuron projection; photoreceptor inner segment; brush border membrane; postsynaptic density; dendritic spine; postsynaptic membrane; growth cone; photoreceptor outer segment; cell soma; apical plasma membrane; plasma membrane; presynaptic active zone; neurofilament; ionotropic glutamate receptor complex; cell junction

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; protein binding; SH3 domain binding

Biological Process: synaptogenesis; adult behavior; brain development; learning; social behavior; response to nutrient; memory

Research Articles on Shank2

1. Shank2E is concentrated in the apical domain of renal PT cells, specifically binds NaP(i)-IIa via PDZ interactions, and undergoes P(i)-induced internalization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Shank2 antibody

Products by Pathway	Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram

Diseases associated with anti-Shank2 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>17 publications with Shank2 and Nervous System Diseases
Intellectual Disability Antibodies	>13 publications with Shank2 and Intellectual Disability
Autistic Disorder Antibodies	>10 publications with Shank2 and Autistic Disorder
Brain Diseases Antibodies	>7 publications with Shank2 and Brain Diseases
Congenital Abnormalities Antibodies	>5 publications with Shank2 and Congenital Abnormalities
Carcinoma, Squamous Cell Antibodies	>5 publications with Shank2 and Carcinoma, Squamous Cell
Fibrosis Antibodies	>4 publications with Shank2 and Fibrosis
Gastrointestinal Diseases Antibodies	>3 publications with Shank2 and Gastrointestinal Diseases
Diarrhea Antibodies	>3 publications with Shank2 and Diarrhea
Eye Diseases Antibodies	>2 publications with Shank2 and Eye Diseases

Organs/Tissues associated with anti-Shank2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>56 publications with Shank2 and Brain
Kidney Antibodies	>5 publications with Shank2 and Kidney