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DUX4L1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800198

Background Info: This antibody recognizes human DUX4. It does not cross-react with DUX4c.Scientific Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically… More details

$510 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DUX4 (DUX4L1), Monoclonal Antibody
Full Product Name
DUX4 Antibody
Product Gene Name
Product Synonym Names
Double homeobox 4; Double homeobox 10; DUX10; Double homeobox protein 4/10
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P2B1
Host
Mouse
Species Reactivity
Human
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Matching Pairs
Unconjugated Antibody: DUX4 Clone #P2B1 (MBS800198)
ATTO 594 Conjugated Antibody: DUX4 Clone #P2B1 (MBS800794)
Molecular Weight
51,090 Da
3D Structure
ModBase 3D Structure for F5GZ66
Certificate of Analysis
1ug/mL was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-DUX4L1 antibody

Applications Tested/Suitable for anti-DUX4L1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-DUX4L1 antibody
1:1000 (WB)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-DUX4L1 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Tissue: C2C12 myoblast cells. Species: Mouse. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Cells transfected with pCS2+DUX4.
Mouse Anti-DUX4L1 Monoclonal Antibody – Cat# MBS800198

Western Blot (WB) of anti-DUX4L1 antibody

Western Blot analysis of Mouse C2C12 cell lysate showing detection of DUX4 protein using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Cells transfected with pCS2+DUX4 which, contains an additional upstream start site.
Mouse Anti-DUX4L1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS800198

Product References and Citations for anti-DUX4L1 antibody

1. Entrez Gene: "Dux4 Double Homeobox, 4" 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA. 104(46): 18157-18162. 3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17(8): 611-623. 4. Lemmers R., et al. (2010) Science Express. 329(5999): 1650-1653. 5. Snider L., et al. (2010) PLoS Genetics. 6:1-14.

Other Notes

Small volumes of anti-DUX4L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DUX4L1 purchase

MBS800198 is an antibody targeting DUX4L1. Also known as DUX4 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DUX4L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
double homeobox protein 4
NCBI Official Synonym Full Names
double homeobox 4 like 1
NCBI Official Symbol
DUX4L1
NCBI Official Synonym Symbols
DUX4; DUX10
UniProt Protein Name
Double homeobox protein 4
UniProt Synonym Protein Names
Double homeobox protein 4; Double homeobox protein 4-like protein 2; Double homeobox protein 4-like protein 3; Double homeobox protein 4-like protein 5; Double homeobox protein 4-like protein 6; Double homeobox protein 4-like protein 7
UniProt Gene Name
DUX4L2
UniProt Synonym Gene Names
DUX4; DUX4L3; DUX4L5; DUX4L6; DUX4L7
UniProt Entry Name
F5GZ66_HUMAN

NCBI Summary for DUX4L1

This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length and a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that encodes two homeoboxes; the repeat-array and ORF is conserved in other mammals. There is no evidence for transcription of the gene at this locus though RT-PCR and in vitro expression experiments indicate that a telomeric paralog of this gene is transcribed in some haplotypes. Contraction of the macrosatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). [provided by RefSeq, Jun 2014]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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