Nitrotyrosine Antibody – Mouse Monoclonal

Background Info: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Scientific Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.
1. Kim. H. et al. (2010). SIRT3 Is a Mitochondria-Localized Tumor Suppressor Required for Maintenance of Mitochondrial Integrity and Metabolism during Stress. Cancer Cell. 17 (1), 41-52. doi:10.1016/j.ccr.2009.11.023

2. Grutzmacher, C. et al. (2012). Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes. AJP Renal Physiol. 304 (1), F19-F30. doi: 10.‹1152/‹ajprenal.‹00036.‹2012
3. Nakayama, T., et al. (2014). Improved survival rate by temperature control at compression sites in rat model of crush syndrome. Journal of Surgical Research. doi: 10.1016/j.jss.2013.12.012
4. Chao, T.H., Chang, M.Y., Su S.J., Su, S.H. (2014). Inducible nitric oxide synthase mediates MG132 lethality in leukemic cells through mitochondrial depolarization. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2014.05.023

Small volumes of anti-Nitrotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS800408 is an antibody targeting Nitrotyrosine. Also known as Nitrotyrosine Antibody: ATTO 390. Reactive in Human, Mouse, Rat, Dog. Host: Mouse.