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HCN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800425

MBS800425 is an antibody targeting Hcn1. Also known as HCN1 Antibody: ATTO 390. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HCN1, Monoclonal Antibody
Full Product Name
HCN1 Antibody: ATTO 390
Product Gene Name
Product Synonym Names
BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC2; HCN1; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S70
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Purity/Purification
Protein G purified
Concentration
1mg/mL (lot specific)
Specificity
Detects ~100kDa. No cross reactivity with HCN2.
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS800425)
ATTO 390 Conjugated Antibody: HCN1 Clone #S70-28 (MBS800425)
Matching Pairs
Unconjugated Antibody: HCN1 Clone #S70-28 (MBS803623)
ATTO 390 Conjugated Antibody: HCN1 Clone #S70-28 (MBS800425)
3D Structure
ModBase 3D Structure for Q9JKBO
Conjugate
ATTO 390
Immunogen
Fusion protein amino acids 778-910 (C-terminus) of rat HCN1.
Cellular Localization
Membrane
Research Area(s)
Neuroscience , Ion Channels, Cyclic Necleotide-Gated Ion Channels, Cardiovascular System, Heart
Function
Hyperpolarization-activated cation channels of the HCN gene family , such as HCN1, play a crucial role in the regulations of cell excitability. Importantly , they contribute to spontaneous rhythmic activity in both the heart and brain.

Product Categories/Family for anti-Hcn1 antibody

Applications Tested/Suitable for anti-Hcn1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemsitry (ICC)
Application Notes for anti-Hcn1 antibody
WB: 1:1000
IHC: 1:1000
ICC/IF: 1:100
Optimal dilutions for assays should be determined by the user.

Immunohistochemistry (IHC) of anti-Hcn1 antibody

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.
Mouse Anti-HCN1 Monoclonal Antibody – Cat# MBS800425

Western Blot (WB) of anti-Hcn1 antibody

Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-HCN1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS800425

Immunohistochemistry (IHC) of anti-Hcn1 antibody

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Mouse Anti-HCN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS800425

Immunohistochemistry (IHC) of anti-Hcn1 antibody

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.
Mouse Anti-HCN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS800425

Immunohistochemistry (IHC) of anti-Hcn1 antibody

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Mouse Anti-HCN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS800425

Product References and Citations for anti-Hcn1 antibody

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232.

Other Notes

Small volumes of anti-Hcn1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hcn1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Official Synonym Full Names
hyperpolarization-activated cyclic nucleotide-gated potassium channel 1
NCBI Official Symbol
Hcn1
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Gene Name
Hcn1
UniProt Entry Name
HCN1_RAT
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

NCBI Summary for Hcn1

may generate neuronal pacemaker activity [RGD, Feb 2006]

UniProt Comments for Hcn1

HCN1: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. Belongs to the potassium channel HCN family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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