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SCN10A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800796

Background Info: Detects ~220kDa. No cross reactivity against other Nav channelsScientific Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for… More details

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Product Overview

Online Datasheet in PDF
Product Name
Nav1.8 (Scn10a), Monoclonal Antibody
Full Product Name
Nav1.8 Antibody: RPE
Product Gene Name
Product Synonym Names
PN3; Na(V)1.8; Scn10a
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S134-12
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: Nav1.8 Clone #S134-12 (MBS800264)
RPE Conjugated Antibody: Nav1.8 Clone #S134-12 (MBS800796)
Matching Pairs
Unconjugated Antibody: N/A (MBS800796)
RPE Conjugated Antibody: Nav1.8 Clone #S134-12 (MBS800796)
Molecular Weight
219,605 Da
3D Structure
ModBase 3D Structure for Q62968
Conjugate
RPE
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Scn10a antibody

Applications Tested/Suitable for anti-Scn10a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Scn10a antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Scn10a antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.
Mouse Anti-SCN10A Monoclonal Antibody – Cat# MBS800796

Western Blot (WB) of anti-Scn10a antibody

Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-SCN10A Monoclonal Antibody, Western Blot (WB) – Cat# MBS800796

Immunohistochemistry (IHC) of anti-Scn10a antibody

Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.
Mouse Anti-SCN10A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS800796

Product References and Citations for anti-Scn10a antibody

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58. 4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212. 5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.

Other Notes

Small volumes of anti-Scn10a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Scn10a purchase

MBS800796 is an antibody targeting Scn10a. Also known as Nav1.8 Antibody: RPE. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Scn10a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sodium channel protein type 10 subunit alpha
NCBI Official Synonym Full Names
sodium channel, voltage-gated, type X, alpha subunit
NCBI Official Symbol
Scn10a
NCBI Official Synonym Symbols
PN3; Na(V)1.8
UniProt Protein Name
Sodium channel protein type 10 subunit alpha
UniProt Synonym Protein Names
Peripheral nerve sodium channel 3; PN3; Sensory neuron sodium channel; Sodium channel protein type X subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.8
UniProt Gene Name
Scn10a
UniProt Synonym Gene Names
Sns; PN3
UniProt Entry Name
SCNAA_RAT
NCBI Protein Information
sodium channel protein type 10 subunit alpha; sensory neuron sodium channel; peripheral nerve sodium channel 3; sodium channel type X alpha polypeptide; sodium channel protein type X subunit alpha; voltage-gated sodium channel subunit alpha Nav1.8; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage-gated, type 10, alpha polypeptide

NCBI Summary for Scn10a

voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

UniProt Comments for Scn10a

SCN10A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, sodium
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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