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CLCN3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800964

Background Info: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Clcn3, Monoclonal Antibody
Full Product Name
Clcn3 Antibody: ATTO 488
Product Gene Name
Product Synonym Names
CIC3; CLC3; CLCN 3; Chloride channel 3; Chloride channel protein 3; Chloride channel; voltage-sensitive 3; Chloride transporter ClC 3; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; Chloride transporter ClC-3; ClC-3; H(+)/Cl(-) exchange transporter 3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S258-5
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS800964)
ATTO 488 Conjugated Antibody: Clcn3 Clone #S258-5 (MBS800964)
Matching Pairs
Unconjugated Antibody: Clcn3 Clone #S258-5 (MBS802719)
ATTO 488 Conjugated Antibody: Clcn3 Clone #S258-5 (MBS800964)
Molecular Weight
84,461 Da
3D Structure
ModBase 3D Structure for P51792
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Clcn3 antibody

Applications Tested/Suitable for anti-Clcn3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-Clcn3 antibody
1ug/mL (WB), 0.1-1ug/mL (IHC)

Western Blot (WB) of anti-Clcn3 antibody

Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.
Mouse Anti-CLCN3 Monoclonal Antibody – Cat# MBS800964

Product References and Citations for anti-Clcn3 antibody

1.  www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Other Notes

Small volumes of anti-Clcn3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Clcn3 purchase

MBS800964 is an antibody targeting Clcn3. Also known as Clcn3 Antibody: ATTO 488. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Clcn3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
H(+)/Cl(-) exchange transporter 3
NCBI Official Synonym Full Names
chloride channel, voltage-sensitive 3
NCBI Official Symbol
Clcn3
NCBI Official Synonym Symbols
ClC-3
UniProt Protein Name
H(+)/Cl(-) exchange transporter 3
UniProt Synonym Protein Names
Chloride channel protein 3; ClC-3; Chloride transporter ClC-3
UniProt Gene Name
Clcn3
UniProt Synonym Gene Names
ClC-3
UniProt Entry Name
CLCN3_RAT
NCBI Protein Information
H(+)/Cl(-) exchange transporter 3; H(+)/Cl(-) exchange transporter 3; chloride channel 3; chloride channel protein 3; chloride transporter ClC-3; protein kinase C-regulated chloride channel

NCBI Summary for Clcn3

mediates transepithelial chloride transport [RGD, Feb 2006]

UniProt Comments for Clcn3

CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory (By similarity)

Protein type: Channel, chloride; Transporter, ion channel; Vesicle; Transporter; Membrane protein, multi-pass; Membrane protein, integral
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Clcn3 antibody

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