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Ubiquitin (UBB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS800981

Background Info: Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.Scientific Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ubiquitin (UBB), Monoclonal Antibody
Full Product Name
Ubiquitin Antibody: HRP
Product Gene Name
Product Synonym Names
Polyubiquitin B; RPS27A; UBA52; UBB; UBC; ubiquitin B
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FK2
Host
Mouse
Species Reactivity
Wide range of species
Form/Format
Protein A Purified
Concentration
10mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS800981)
HRP Conjugated Antibody: Ubiquitin Clone #FK2 (MBS800981)
Matching Pairs
Unconjugated Antibody: Ubiquitin Clone #FK2 (MBS802572)
HRP Conjugated Antibody: Ubiquitin Clone #FK2 (MBS800981)
Molecular Weight
25,762 Da
OMIM
191339
3D Structure
ModBase 3D Structure for P0CG47
Conjugate
HRP
Storage Buffer
10mM phosphate buffer with 0.15M NaCl, pH7.4, 0.1% sodium azide

Product Categories/Family for anti-UBB antibody

Applications Tested/Suitable for anti-UBB antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-UBB antibody
1:1000-10000 (WB)

Immunocytochemistry (ICC) of anti-UBB antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
Mouse Anti-UBB Monoclonal Antibody – Cat# MBS800981

Product References and Citations for anti-UBB antibody

1. Chen, J., & Chen, Z. (2013). Regulation of NF-kappaB by ubiquitination. Curr Opin Immunol, (1):4-12. 2. Shaid, S., Brandts, C., Serve, H., & Dikic, I. (2013). Ubiquitination and selective autophagy. Cell Death Differ, 20(1):21-30.

Other Notes

Small volumes of anti-UBB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBB purchase

MBS800981 is an antibody targeting UBB. Also known as Ubiquitin Antibody: HRP. Reactive in Wide range of species. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for UBB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
polyubiquitin B
NCBI Official Synonym Full Names
ubiquitin B
NCBI Official Symbol
UBB
UniProt Protein Name
Polyubiquitin-B
Protein Family
UniProt Gene Name
UBB
UniProt Entry Name
UBB_HUMAN
NCBI Protein Information
polyubiquitin-B; polyubiquitin B

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-UBB antibody

 Products by Pathway  Pathway Diagram
 AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies  AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram
 APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway antibodies  APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway Diagram
 APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies  APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 AUF1 (hnRNP D0) Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Destabilizes MRNA Pathway Diagram
 AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies  AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram
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