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NALCN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS801201

Background Info: Detects ~200kDa.Scientific Background: NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NALCN, Monoclonal Antibody
Full Product Name
NALCN Antibody: ATTO 488
Product Gene Name
Product Synonym Names
VGCNL1; CanIon; Sodium leak channel non-selective protein; Voltage gated channel-like protein 1; A530023G15Rik; bA430M15.1; FLJ23913; FLJ44659; FLJ44764; Four repeat voltage gated ion channel; MGC74524; Putative 4 repeat voltage gated ion channel
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S185-7
Host
Mouse
Species Reactivity
Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS801201)
ATTO 488 Conjugated Antibody: NALCN Clone #S185-7 (MBS801201)
Matching Pairs
Unconjugated Antibody: NALCN Clone #S185-7 (MBS801264)
ATTO 488 Conjugated Antibody: NALCN Clone #S185-7 (MBS801201)
Molecular Weight
200,491 Da
3D Structure
ModBase 3D Structure for Q6Q760
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Nalcn antibody

Applications Tested/Suitable for anti-Nalcn antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Nalcn antibody
1ug/mL (WB)

Immunocytochemistry (ICC) of anti-Nalcn antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.
Mouse Anti-NALCN Monoclonal Antibody – Cat# MBS801201

Western Blot (WB) of anti-Nalcn antibody

Western Blot analysis of Rat Brain showing detection of ~200 kDa NALCN protein using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 . Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Mouse Anti-NALCN Monoclonal Antibody, Western Blot (WB) – Cat# MBS801201

Product References and Citations for anti-Nalcn antibody

1. Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236. 2. Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383. 3. Jospin, M., et al. (2007) Curr. Biol. 17: 1595-1600. 4. Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530. 5. Yeh, E., et al. (2008) PLoS Biol. 6: e55.

Other Notes

Small volumes of anti-Nalcn antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nalcn purchase

MBS801201 is an antibody targeting Nalcn. Also known as NALCN Antibody: ATTO 488. Reactive in Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Nalcn. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sodium leak channel non-selective protein
NCBI Official Synonym Full Names
sodium leak channel, non-selective
NCBI Official Symbol
Nalcn
NCBI Official Synonym Symbols
Vgcnl1
UniProt Protein Name
Sodium leak channel non-selective protein
UniProt Synonym Protein Names
Four domain-type voltage-gated ion channel alpha-1 subunit; Voltage gated channel-like protein 1
UniProt Gene Name
Nalcn
UniProt Synonym Gene Names
Nca; Vgcnl1
UniProt Entry Name
NALCN_RAT
NCBI Protein Information
sodium leak channel non-selective protein; voltage gated channel like 1; brain voltage-gated cation channel; voltage gated channel-like protein 1; four domain-type voltage-gated ion channel alpha-1 subunit

NCBI Summary for Nalcn

may act as a voltage activated cation channel [RGD, Feb 2006]

UniProt Comments for Nalcn

VGCNL1: a putative voltage-gated ion channel

Protein type: Channel, calcium; Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: plasma membrane; integral to membrane

Molecular Function: voltage-gated calcium channel activity; voltage-gated ion channel activity; cation channel activity; sodium channel activity

Biological Process: sperm motility
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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