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CAMK2A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS801337

Background Info: Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).Scientific Background: CaMKII is an important member of the… More details

$535 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CaMKII (Camk2a), Monoclonal Antibody
Full Product Name
CaMKII Antibody: FITC
Product Gene Name
anti-Camk2a antibody
Product Synonym Names
CamK2; CamK2A; CamK2B; CamK2D; CamK2G; CAMKA
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
22B1
Host
Mouse
Species Reactivity
Rat, Human
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: CaMKII Clone #22B1 (MBS804370)
FITC Conjugated Antibody: CaMKII Clone #22B1 (MBS801337)
Molecular Weight
54,115 Da
3D Structure
ModBase 3D Structure for P11275
Conjugate
FITC
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Camk2a antibody

Cell Signaling
Applications Tested/Suitable for anti-Camk2a antibody
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-Camk2a antibody
1ug/ml was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Camk2a antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Mouse Anti-CAMK2A Monoclonal Antibody – Cat# MBS801337

Immunohistochemistry (IHC) of anti-Camk2a antibody

Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.
Mouse Anti-CAMK2A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS801337

Immunohistochemistry (IHC) of anti-Camk2a antibody

Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.
Mouse Anti-CAMK2A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS801337

Western Blot (WB) of anti-Camk2a antibody

Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.
Mouse Anti-CAMK2A Monoclonal Antibody, Western Blot (WB) – Cat# MBS801337

Product References and Citations for anti-Camk2a antibody

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008-36013. 2. Barria A. et al. (1997) Science 276: 2042-2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4-12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500-2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
1. Feng, T., Szabo, E., Dziak, E. and Opas, M. (2010). Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells. Stem Cell Rev. 6 (1), 74-85. doi: 10.1007/s12015-010-9115-8.

Other Notes

Small volumes of anti-Camk2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Camk2a purchase

MBS801337 is an antibody targeting Camk2a. Also known as CaMKII Antibody: FITC. Reactive in Rat, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Camk2a. It may not necessarily be applicable to this product.

NCBI GI #
6978593
NCBI GeneID
25400
NCBI Accession #
NP_037052.1
UniProt Primary Accession #
P11275
UniProt Related Accession #
P11275
GenBank Nucleotide #
NM_012920.1
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type II subunit alpha
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase II alpha
NCBI Official Symbol
Camk2a
NCBI Official Synonym Symbols
PKCCD; PK2CDD
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Protein Family
Calcium/calmodulin-dependent protein kinase
UniProt Gene Name
Camk2a
UniProt Synonym Gene Names
CaM kinase II subunit alpha; CaMK-II subunit alpha
UniProt Entry Name
KCC2A_RAT
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha

UniProt Comments for Camk2a

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; CAMK2 family

Cellular Component: dendrite cytoplasm; presynaptic membrane; cell soma; mitochondrion; axon; postsynaptic density; dendrite; nucleus; cell junction

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; GTPase activating protein binding; kinase activity; ATP binding

Biological Process: peptidyl-serine phosphorylation; ionotropic glutamate receptor signaling pathway; calcium ion transport; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity
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Research Articles on Camk2a

1. Increased Camk2a autophosphorylation was found in the medial prefrontal cortex and nucleus accumbens after last cocaine self-administration session.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Camk2a antibody

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 Camk2b antibody  >7 publications with Camk2a and Camk2b
 Arc antibody  >2 publications with Camk2a and Arc
 Grin2a antibody  >1 publications with Camk2a and Grin2a

Pathways associated with anti-Camk2a antibody

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 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
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 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram

Diseases associated with anti-Camk2a antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >48 publications with Camk2a and Nervous System Diseases
 Brain Diseases Antibodies  >32 publications with Camk2a and Brain Diseases
 Seizures Antibodies  >8 publications with Camk2a and Seizures
 Pain Antibodies  >8 publications with Camk2a and Pain
 Memory Disorders Antibodies  >6 publications with Camk2a and Memory Disorders
 Movement Disorders Antibodies  >6 publications with Camk2a and Movement Disorders
 Learning Disorders Antibodies  >5 publications with Camk2a and Learning Disorders
 Inflammation Antibodies  >5 publications with Camk2a and Inflammation
 Cognition Disorders Antibodies  >4 publications with Camk2a and Cognition Disorders
 Necrosis Antibodies  >3 publications with Camk2a and Necrosis

Organs/Tissues associated with anti-Camk2a antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >138 publications with Camk2a and Brain
 Muscle Antibodies  >5 publications with Camk2a and Muscle
 Embryonic Tissue Antibodies  >4 publications with Camk2a and Embryonic Tissue
 Heart Antibodies  >3 publications with Camk2a and Heart
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