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DNMT3L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS801702

Background Info: Detects ~45kDa. No cross-reactivity against DNMT3A or DNMT3B.Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian… More details

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Product Overview

Product Name
Dnmt3L, Monoclonal Antibody
Full Product Name
Dnmt3L Antibody: Alkaline Phosphatase
Product Gene Name
anti-DNMT3L antibody
Product Synonym Names
DNA (cytosine-5-)-methyltransferease 3-like; MGC1090
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S117-9
Host
Mouse
Species Reactivity
Human
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
43,670 Da
OMIM
606588
3D Structure
ModBase 3D Structure for Q9UJW3
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-DNMT3L antibody

Epigenetics
Applications Tested/Suitable for anti-DNMT3L antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-DNMT3L antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunohistochemistry (IHC) of anti-DNMT3L antibody

Immunohistochemistry analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower layers of the epidermis have positive cell staining in the cytoplasm.
Mouse Anti-DNMT3L Monoclonal Antibody – Cat# MBS801702

Western Blot (WB) of anti-DNMT3L antibody

Western Blot analysis of Human tissue lysate showing detection of Dnmt3L protein using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-DNMT3L Monoclonal Antibody, Western Blot (WB) – Cat# MBS801702

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-DNMT3L antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Staining in the nucleus, and accumulated around the nucleus. Clear nuclear demarcations.
Mouse Anti-DNMT3L Monoclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS801702

Product References and Citations for anti-DNMT3L antibody

1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S. et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342. 7. Kareta MS., et al. (2006) J Biol Chem. 281(36): 25893- 25902. 8. Gokul G., Ramakrishna G., and Khosla S. (2009) Epigenetics. 4(5): 322-329.

Other Notes

Small volumes of anti-DNMT3L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNMT3L purchase

MBS801702 is an antibody targeting DNMT3L. Also known as Dnmt3L Antibody: Alkaline Phosphatase. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DNMT3L. It may not necessarily be applicable to this product.

NCBI GI #
28872778
NCBI GeneID
29947
NCBI Accession #
NP_037501.2
UniProt Primary Accession #
Q9UJW3
UniProt Secondary Accession #
Q9BUJ4; E9PB42
UniProt Related Accession #
Q9UJW3
GenBank Nucleotide #
NM_013369.3
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3-like isoform 1
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3-like
NCBI Official Symbol
DNMT3L
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3-like
Protein Family
DNA (cytosine-5)-methyltransferase
UniProt Gene Name
DNMT3L
UniProt Entry Name
DNM3L_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein

NCBI Summary for DNMT3L

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
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UniProt Comments for DNMT3L

DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity

Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; gene expression; spermatogenesis; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; DNA methylation on cytosine
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Research Articles on DNMT3L

1. DNMT3L is one of the key players in de novo DNA methylation of imprinting control elements and retrotransposons, which occurs after genome-wide epigenetic erasure during germ cell development. (Review)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DNMT3L antibody

 Reference Product  PubMed Publications
 DNMT3A antibody  >114 publications with DNMT3L and DNMT3A
 DNMT3B antibody  >84 publications with DNMT3L and DNMT3B
 MAT2B antibody  >1 publications with DNMT3L and MAT2B
 MAT2A antibody  >1 publications with DNMT3L and MAT2A

Pathways associated with anti-DNMT3L antibody

 Products by Pathway  Pathway Diagram
 Trans-sulfuration And One Carbon Metabolism Pathway antibodies  Trans-sulfuration And One Carbon Metabolism Pathway Diagram

Diseases associated with anti-DNMT3L antibody

 Disease Name  Pubmed Publications
 Genital Diseases, Male Antibodies  >14 publications with DNMT3L and Genital Diseases, Male
 Infertility, Male Antibodies  >12 publications with DNMT3L and Infertility, Male
 Testicular Diseases Antibodies  >7 publications with DNMT3L and Testicular Diseases
 Congenital Abnormalities Antibodies  >5 publications with DNMT3L and Congenital Abnormalities
 Chromosome Aberrations Antibodies  >5 publications with DNMT3L and Chromosome Aberrations
 Cell Transformation, Neoplastic Antibodies  >4 publications with DNMT3L and Cell Transformation, Neoplastic
 Infertility, Female Antibodies  >4 publications with DNMT3L and Infertility, Female
 Oligospermia Antibodies  >3 publications with DNMT3L and Oligospermia
 Uterine Neoplasms Antibodies  >3 publications with DNMT3L and Uterine Neoplasms
 Adenocarcinoma Antibodies  >3 publications with DNMT3L and Adenocarcinoma

Organs/Tissues associated with anti-DNMT3L antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >27 publications with DNMT3L and Testis
 Embryonic Tissue Antibodies  >20 publications with DNMT3L and Embryonic Tissue
 Placenta Antibodies  >9 publications with DNMT3L and Placenta
 Mixed Antibodies  >1 publications with DNMT3L and Mixed
 Epididymis Antibodies  >1 publications with DNMT3L and Epididymis
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