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Brevican (BCAN) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS801883

Background Info: Detects ~140kDa (and smaller due to proteolytic cleavage)Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Brevican (Bcan), Monoclonal Antibody
Full Product Name
Brevican Antibody: Biotin
Product Gene Name
Product Synonym Names
BCAN; BEHAB; CSPG7; Brain enriched hyaluronan binding protein antibody; Brevican core protein; Brevican core protein isoform 1; Brevican core protein isoform 2; Brevican proteoglycan; Chondroitin sulfate proteoglycan 7
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
S294A-6
Host
Mouse
Species Reactivity
Rat, Mouse
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: Brevican Clone #S294A-6 (MBS800457)
Biotin Conjugated Antibody: Brevican Clone #S294A-6 (MBS801883)
Matching Pairs
Unconjugated Antibody: N/A (MBS801883)
Biotin Conjugated Antibody: Brevican Clone #S294A-6 (MBS801883)
Molecular Weight
69,085 Da
3D Structure
ModBase 3D Structure for P55068
Conjugate
Biotin
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Bcan antibody

Applications Tested/Suitable for anti-Bcan antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-Bcan antibody
1:1000 (WB)

Western Blot (WB) of anti-Bcan antibody

Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.
Mouse Anti-BCAN Monoclonal Antibody – Cat# MBS801883

Product References and Citations for anti-Bcan antibody

1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126. 2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M. and Yamaguchi Y. (1995) Biochem. Biophys. Res. Commun. 216: 957-963. 3. Rauch U., et al. (1997) Genomics 44: 15-21. 4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and Hockfield S. (1998) J. Neurosci. 7: 2370-2376. 5. Aspberg A., Adam S., Kostka G., Timpl R. and Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449.

Other Notes

Small volumes of anti-Bcan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bcan purchase

MBS801883 is an antibody targeting Bcan. Also known as Brevican Antibody: Biotin. Reactive in Rat, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Bcan. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
brevican core protein isoform 1
NCBI Official Synonym Full Names
brevican
NCBI Official Symbol
Bcan
NCBI Official Synonym Symbols
BEHAB; ALPBRE
UniProt Protein Name
Brevican core protein
UniProt Synonym Protein Names
Brain-enriched hyaluronan-binding protein; BEHAB
Protein Family
UniProt Gene Name
Bcan
UniProt Synonym Gene Names
Behab; BEHAB
UniProt Entry Name
PGCB_RAT
NCBI Protein Information
brevican core protein; brain-enriched hyaluronan-binding protein; brevican (brain specific proteoglycan in the aggrecan family)

NCBI Summary for Bcan

brain-specific member of the aggrecan family of chondroitin sulfate proteoglycans; extracellular matrix (ECM) protein; binds hyaluronan, a ubiquitous component of the ECM [RGD, Feb 2006]

UniProt Comments for Bcan

BCAN: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Belongs to the aggrecan/versican proteoglycan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; dendrite; synapse

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: central nervous system development; hippocampus development; cell adhesion; skeletal development; regulation of neuronal synaptic plasticity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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