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SLC12A5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS801940

Background Info: Detects ~140kDaScientific Background: KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KCC2 (Slc12a5), Monoclonal Antibody
Full Product Name
KCC2 Antibody: ATTO 594
Product Gene Name
anti-Slc12a5 antibody
Product Synonym Names
Potassium Chloride Cotransporter; Potassium chloride transporter 5; SLC12A5; hKCC2; S12A5; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; rKCC2
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S1-12
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: KCC2 Clone #S1-12 (MBS800736)
ATTO 594 Conjugated Antibody: KCC2 Clone #S1-12 (MBS801940)
Matching Pairs
Unconjugated Antibody: N/A (MBS801940)
ATTO 594 Conjugated Antibody: KCC2 Clone #S1-12 (MBS801940)
Molecular Weight
123,563 Da
3D Structure
ModBase 3D Structure for Q63633
Conjugate
ATTO 594
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide.

Product Categories/Family for anti-Slc12a5 antibody

Transporters
Applications Tested/Suitable for anti-Slc12a5 antibody
Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-Slc12a5 antibody
1:300 (IHC)

Western Blot (WB) of anti-Slc12a5 antibody

Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:1000.
Mouse Anti-SLC12A5 Monoclonal Antibody – Cat# MBS801940

Product References and Citations for anti-Slc12a5 antibody

1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.

Other Notes

Small volumes of anti-Slc12a5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Slc12a5 purchase

MBS801940 is an antibody targeting Slc12a5. Also known as KCC2 Antibody: ATTO 594. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Slc12a5. It may not necessarily be applicable to this product.

NCBI GI #
19705463
NCBI GeneID
171373
NCBI Accession #
NP_599190
UniProt Primary Accession #
Q63633
UniProt Secondary Accession #
A7Y821
UniProt Related Accession #
Q63633
GenBank Nucleotide #
NM_134363.1
NCBI Official Full Name
solute carrier family 12 member 5
NCBI Official Synonym Full Names
solute carrier family 12 (potassium-chloride transporter), member 5
NCBI Official Symbol
Slc12a5
NCBI Official Synonym Symbols
Kcc2
UniProt Protein Name
Solute carrier family 12 member 5
UniProt Synonym Protein Names
Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; rKCC2; Neuronal K-Cl cotransporter
Protein Family
Solute carrier family
UniProt Gene Name
Slc12a5
UniProt Synonym Gene Names
Kcc2; rKCC2
UniProt Entry Name
S12A5_RAT
NCBI Protein Information
solute carrier family 12 member 5; rKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; solute carrier family 12, member 5; neuronal-specific K-Cl cotransporter; furosemide-sensitive K-Cl cotransporter; electroneutral potassium-chloride cotransporter 2

NCBI Summary for Slc12a5

neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD, Feb 2006]

UniProt Comments for Slc12a5

KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Cellular Component: cell soma; integral to plasma membrane; plasma membrane; perikaryon; dendritic shaft

Molecular Function: protein binding; ammonium transmembrane transporter activity; protein kinase binding; potassium:chloride symporter activity

Biological Process: response to drug; synaptic transmission; multicellular organism growth; intracellular pH reduction; chloride transport; learning; potassium ion transport; ammonium transport; thermosensory behavior
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Research Articles on Slc12a5

1. Exercise returns spinal excitability and levels of KCC2 toward normal levels in the lumbar spinal cord.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Slc12a5 antibody

 Products by Pathway  Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram

Diseases associated with anti-Slc12a5 antibody

 Disease Name  Pubmed Publications
 Epilepsy Antibodies  >3 publications with Slc12a5 and Epilepsy
 Growth Disorders Antibodies  >2 publications with Slc12a5 and Growth Disorders
 Cognition Disorders Antibodies  >2 publications with Slc12a5 and Cognition Disorders
 Seizures Antibodies  >2 publications with Slc12a5 and Seizures
 Hypertension Antibodies  >1 publications with Slc12a5 and Hypertension
 Liver Diseases Antibodies  >1 publications with Slc12a5 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with Slc12a5 and Kidney Diseases
 Necrosis Antibodies  >1 publications with Slc12a5 and Necrosis

Organs/Tissues associated with anti-Slc12a5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with Slc12a5 and Brain
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