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Erp57 (Grp58) (PDIA3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS803258

Background Info: Detects ~57kDa.Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family,… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Erp57 (Grp58) (PDIA3), Monoclonal Antibody
Full Product Name
Erp57 (Grp58) Antibody: PerCP
Product Gene Name
anti-PDIA3 antibody
Product Synonym Names
ERp60; ERp61; Grp57; Grp58; P58; PDIA3; PI PLC
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Map.ERP57
Host
Mouse
Species Reactivity
Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
56,782 Da
OMIM
602046
3D Structure
ModBase 3D Structure for P30101
Conjugate
PerCP
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-PDIA3 antibody

Heat Shock; Organelle Markers
Applications Tested/Suitable for anti-PDIA3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-PDIA3 antibody
1:2000 (WB)

Immunohistochemistry (IHC) of anti-PDIA3 antibody

Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.
Mouse Anti-PDIA3 Monoclonal Antibody – Cat# MBS803258

Western Blot (WB) of anti-PDIA3 antibody

Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-PDIA3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS803258

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-PDIA3 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.
Mouse Anti-PDIA3 Monoclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS803258

Product References and Citations for anti-PDIA3 antibody

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
1. Lynes, E.M. et al. (2013). Palmitoylation is the Switch that Assigns Calnexin to Quality Control or ER Calcium Signaling. J Cell Sci. Advance Online Article July 10, 2013. doi: 10.1242/jcs.125856

Other Notes

Small volumes of anti-PDIA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA3 purchase

MBS803258 is an antibody targeting PDIA3. Also known as Erp57 (Grp58) Antibody: PerCP. Reactive in Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA3. It may not necessarily be applicable to this product.

NCBI GI #
21361657
NCBI GeneID
2923
NCBI Accession #
NP_005304.3
UniProt Primary Accession #
P30101
UniProt Secondary Accession #
Q13453; Q14255; Q8IYF8; Q9UMU7
UniProt Related Accession #
P30101; AAB37397
GenBank Nucleotide #
NM_005313.4
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Synonym Protein Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Protein Family
Protein disulfide-isomerase
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_HUMAN
NCBI Protein Information
protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n

NCBI Summary for PDIA3

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDIA3

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Endoplasmic reticulum; EC 5.3.4.1; Phospholipase; Protease; Chaperone; Apoptosis; Isomerase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: cell surface; smooth endoplasmic reticulum; focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; cell redox homeostasis; protein import into nucleus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; proteolysis; post-translational protein modification; signal transduction; protein retention in ER
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Research Articles on PDIA3

1. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDIA3 antibody

 Reference Product  PubMed Publications
 STAT3 antibody  >12 publications with PDIA3 and STAT3
 HLA-B antibody  >7 publications with PDIA3 and HLA-B
 HLA-A antibody  >4 publications with PDIA3 and HLA-A
 CALR antibody  >3 publications with PDIA3 and CALR
 ERP27 antibody  >1 publications with PDIA3 and ERP27
 CANX antibody  >1 publications with PDIA3 and CANX

Pathways associated with anti-PDIA3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ER-Phagosome Pathway antibodies  ER-Phagosome Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-PDIA3 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >14 publications with PDIA3 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >11 publications with PDIA3 and Cardiovascular Diseases
 Inflammation Antibodies  >10 publications with PDIA3 and Inflammation
 Nervous System Diseases Antibodies  >8 publications with PDIA3 and Nervous System Diseases
 Liver Diseases Antibodies  >8 publications with PDIA3 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with PDIA3 and Liver Neoplasms
 Immune System Diseases Antibodies  >7 publications with PDIA3 and Immune System Diseases
 Brain Diseases Antibodies  >7 publications with PDIA3 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with PDIA3 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >4 publications with PDIA3 and Fibrosis

Organs/Tissues associated with anti-PDIA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with PDIA3 and Blood
 Liver Antibodies  >21 publications with PDIA3 and Liver
 Kidney Antibodies  >14 publications with PDIA3 and Kidney
 Brain Antibodies  >14 publications with PDIA3 and Brain
 Bone Antibodies  >12 publications with PDIA3 and Bone
 Lung Antibodies  >11 publications with PDIA3 and Lung
 Intestine Antibodies  >11 publications with PDIA3 and Intestine
 Muscle Antibodies  >10 publications with PDIA3 and Muscle
 Vascular Antibodies  >8 publications with PDIA3 and Vascular
 Connective Tissue Antibodies  >8 publications with PDIA3 and Connective Tissue
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