LAMP1 Antibody – Mouse Monoclonal

Background Info: Detects an ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.

Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68. 2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76. 4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19. 5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847. 6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.
1. Hiasa, M. et al. (2014). Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets. Physiol Rep. 2(6). pii: e12034. doi: 10.14814/phy2.12034

Small volumes of anti-Lamp1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS803271 is an antibody targeting Lamp1. Also known as LAMP1 Antibody: PerCP. Reactive in Rat, Hamster. Host: Mouse.