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Bk Beta3a (KCNMB3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS804394

Background Info: Detects ~32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.Scientific Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (3).… More details

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Product Overview

Product Name
BK Beta3a (KCNMB3), Monoclonal Antibody
Full Product Name
BK Beta3a Antibody : RPE
Product Gene Name
anti-KCNMB3 antibody
Product Synonym Names
HBETA3; KCNMB2; KCNMBL; BKBeta3; KCNMB3; Calcium-activated potassium channel subunit beta-3; BK channel subunit beta-3; BKbeta3; Hbeta3; Calcium-activated potassium channel; subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3; K(VCA)beta-3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S40B-18
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
19,474 Da
OMIM
605222
3D Structure
ModBase 3D Structure for Q9NPA1
Conjugate
RPE
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-KCNMB3 antibody

Ion Channels; Neuroscience
Applications Tested/Suitable for anti-KCNMB3 antibody
Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-KCNMB3 antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-KCNMB3 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Accumulation at the edges of the cell, ruffling edges.
Mouse Anti-KCNMB3 Monoclonal Antibody – Cat# MBS804394

Western Blot (WB) of anti-KCNMB3 antibody

Western Blot analysis of Rat brain membrane lysate showing detection of KCNMB3 Potassium Channel protein using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Mouse Anti-KCNMB3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS804394

Immunohistochemistry (IHC) of anti-KCNMB3 antibody

Immunohistochemistry analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful filaggrin-like staining.
Mouse Anti-KCNMB3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS804394

Product References and Citations for anti-KCNMB3 antibody

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1- 13. 4. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481- 1489.

Other Notes

Small volumes of anti-KCNMB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KCNMB3 purchase

MBS804394 is an antibody targeting KCNMB3. Also known as BK Beta3a Antibody : RPE. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KCNMB3. It may not necessarily be applicable to this product.

NCBI GI #
25952095
NCBI GeneID
27094
NCBI Accession #
NP_055222.3
UniProt Primary Accession #
Q9NPA1
UniProt Secondary Accession #
Q9NPG7; Q9NRM9; Q9UHN3; B7Z9C9; D3DNR2; E9PER5
UniProt Related Accession #
Q9NPA1
GenBank Nucleotide #
NM_014407.3
NCBI Official Full Name
calcium-activated potassium channel subunit beta-3 isoform d
NCBI Official Synonym Full Names
potassium large conductance calcium-activated channel, subfamily M beta member 3
NCBI Official Symbol
KCNMB3
NCBI Official Synonym Symbols
HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3; SLO-BETA-3; K(VCA)BETA-3
UniProt Protein Name
Calcium-activated potassium channel subunit beta-3
UniProt Synonym Protein Names
BK channel subunit beta-3; BKbeta3; Hbeta3; Calcium-activated potassium channel, subfamily M subunit beta-3; Charybdotoxin receptor subunit beta-3; K(VCA)beta-3; Maxi K channel subunit beta-3; Slo-beta-3
Protein Family
Calcium-activated potassium channel
UniProt Gene Name
KCNMB3
UniProt Synonym Gene Names
KCNMB2; KCNMBL; BKbeta3; Hbeta3
UniProt Entry Name
KCMB3_HUMAN
NCBI Protein Information
calcium-activated potassium channel subunit beta-3; BK channel subunit beta-3; maxi K channel subunit beta-3; charybdotoxin receptor subunit beta-3; calcium-activated potassium channel regulatory subunit; calcium-activated potassium channel, subfamily M subunit beta-3; large conductance, voltage and Ca2+ activated potassium channel Maxi K beta 3 subunit

NCBI Summary for KCNMB3

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22. [provided by RefSeq, Jul 2009]
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UniProt Comments for KCNMB3

KCNMB3: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane

Molecular Function: calcium-activated potassium channel activity; potassium channel regulator activity

Biological Process: regulation of action potential; synaptic transmission; generation of action potential; detection of calcium ion; blood coagulation; potassium ion transport
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Research Articles on KCNMB3

1. Report beta subunit (KNMB1-4)-specific modulations of BK channel function by a Slo1 mutation associated with epilepsy and dyskinesia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KCNMB3 antibody

 Reference Product  PubMed Publications
 KCNMA1 antibody  >3 publications with KCNMB3 and KCNMA1
 CLCN2 antibody  >1 publications with KCNMB3 and CLCN2

Pathways associated with anti-KCNMB3 antibody

 Products by Pathway  Pathway Diagram
 Ca2+ Activated K+ Channels Pathway antibodies  Ca2+ Activated K+ Channels Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Insulin Secretion Pathway antibodies  Insulin Secretion Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Nitric Oxide Stimulates Guanylate Cyclase Pathway antibodies  Nitric Oxide Stimulates Guanylate Cyclase Pathway Diagram
 Platelet Homeostasis Pathway antibodies  Platelet Homeostasis Pathway Diagram
 Potassium Channels Pathway antibodies  Potassium Channels Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 CGMP Effects Pathway antibodies  CGMP Effects Pathway Diagram

Diseases associated with anti-KCNMB3 antibody

 Disease Name  Pubmed Publications
 Abnormalities, Multiple Antibodies  >1 publications with KCNMB3 and Abnormalities, Multiple

Organs/Tissues associated with anti-KCNMB3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with KCNMB3 and Brain
 Testis Antibodies  >2 publications with KCNMB3 and Testis
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