Mouse Anti-SLC17A8 Monoclonal Antibody – Cat# MBS804671

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34 . Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.

SLC17A8 Antibody – Mouse Monoclonal

Name: SLC17A8 Antibody; Monoclonal; Mouse; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS804671
Price: 575 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS804671

Background Info: Detects ~65kDaScientific Background: Vesicular Glutamate Transporter 3 (VGLUT3) is a multipass membrane protein restricted to synaptic vesicles of glutamergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus,… More details

$575 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VGlut3 (Slc17a8), Monoclonal Antibody

Full Product Name

VGlut3 Antibody: ATTO 488

Product Gene Name

anti-Slc17a8 antibody

Product Synonym Names

DFNA25; Vesicular glutamate transporter 3; deafness autosomal dominant 25; SLC17A8; Solute carrier family 17 member 8; VGLUT 3; Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8

Clonality

Monoclonal

Isotype

IgG1

Clone Number

S34-34

Host

Mouse

Species Reactivity

Mouse, Rat

Form/Format

Protein G Purified

Concentration

1mg/mL (lot specific)

Preparation and Storage

-20 degree C

Matching Pairs

Unconjugated Antibody: VGlut3 Clone #S34-34 (MBS803313)
ATTO 488 Conjugated Antibody: VGlut3 Clone #S34-34 (MBS804671)

Matching Pairs

Unconjugated Antibody: N/A (MBS804671)
ATTO 488 Conjugated Antibody: VGlut3 Clone #S34-34 (MBS804671)

Molecular Weight

64,755 Da

3D Structure

ModBase 3D Structure for Q7TSF2

Conjugate

ATTO 488

Storage Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Slc17a8 antibody

Ion Channels; Neuroscience; Transporters

Applications Tested/Suitable for anti-Slc17a8 antibody

Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-Slc17a8 antibody

1:1000 (WB)

Product References and Citations for anti-Slc17a8 antibody

1. Amilhon B., et al. (2010) J Neurosci. 30(6): 2198-2210. 2. Herzog E., et al. (2004) Neurosci. 123(4): 983-1002.

Other Notes

Small volumes of anti-Slc17a8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Slc17a8 purchase

MBS804671 is an antibody targeting Slc17a8. Also known as VGlut3 Antibody: ATTO 488. Reactive in Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Slc17a8. It may not necessarily be applicable to this product.

NCBI GeneID

266767

NCBI Accession #

NP_74947.1

UniProt Primary Accession #

Q7TSF2

UniProt Secondary Accession #

Q8K1Q1

UniProt Related Accession #

Q7TSF2

NCBI Official Synonym Full Names

solute carrier family 17 (vesicular glutamate transporter), member 8

NCBI Official Symbol

Slc17a8

NCBI Official Synonym Symbols

Vglut3

UniProt Protein Name

Vesicular glutamate transporter 3

UniProt Synonym Protein Names

Solute carrier family 17 member 8

Protein Family

Vesicular glutamate transporter

UniProt Gene Name

Slc17a8

UniProt Synonym Gene Names

Vglut3; VGluT3

UniProt Entry Name

VGLU3_RAT

NCBI Protein Information

vesicular glutamate transporter 3

NCBI Summary for Slc17a8

has glutamate transporter activity; may play a role in glutamate transport in synaptic vesicles of cholinergic and serotoninergic neurons [RGD, Feb 2006]

UniProt Comments for Slc17a8

SLC17A8: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Defects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25). DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age- dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged. Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter

Cellular Component: neuron projection; synaptic vesicle membrane; integral to membrane; cell junction

Molecular Function: symporter activity; L-glutamate transmembrane transporter activity

Biological Process: L-glutamate transport; sensory perception of sound; neurotransmitter transport; sodium ion transport

Research Articles on Slc17a8

1. contribution of distinct VGLUT3 and VMAT2 transporter expression induced by BDNF might be one of the mechanisms that BDNF regulates neuropathic pain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.