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AHSA1 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS804688

Background Info: Detects ~38kDa. Can run up to 45kDa on SDS PageScientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2).… More details

$585 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Aha1 (Ahsa1), Monoclonal Antibody
Full Product Name
Aha1 Antibody: Biotin
Product Gene Name
Product Synonym Names
Aha 1; Ahsa1; p38; HspC322; C14orf3; Activator of Hsp90 ATPase; Activator of 90 kDa heat shock protein ATPase homolog 1
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
25F2.D10
Host
Rat
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1.59mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: Aha1 Clone #25F2.D10 (MBS801692)
Biotin Conjugated Antibody: Aha1 Clone #25F2.D10 (MBS804688)
Molecular Weight
38,117 Da
3D Structure
ModBase 3D Structure for Q8BK64
Conjugate
Biotin
Storage Buffer
0.02M potassium phosphate, 0.15M sodium chloride, pH7.2

Product Categories/Family for anti-Ahsa1 antibody

Applications Tested/Suitable for anti-Ahsa1 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Ahsa1 antibody
1:1000 (WB)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Ahsa1 antibody

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).
Rat Anti-AHSA1 Monoclonal Antibody – Cat# MBS804688

Immunoprecipitation (IP) of anti-Ahsa1 antibody

Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.
Rat Anti-AHSA1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS804688

Immunohistochemistry (IHC) of anti-Ahsa1 antibody

Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
Rat Anti-AHSA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS804688

Western Blot (WB) of anti-Ahsa1 antibody

Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Rat Anti-AHSA1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS804688

Product References and Citations for anti-Ahsa1 antibody

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

Other Notes

Small volumes of anti-Ahsa1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ahsa1 purchase

MBS804688 is an antibody targeting Ahsa1. Also known as Aha1 Antibody: Biotin. Reactive in Human, Mouse, Rat. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Ahsa1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
activator of 90 kDa heat shock protein ATPase homolog 1
NCBI Official Synonym Full Names
AHA1, activator of heat shock protein ATPase 1
NCBI Official Symbol
Ahsa1
NCBI Official Synonym Symbols
p38; BC023857
UniProt Protein Name
Activator of 90 kDa heat shock protein ATPase homolog 1
UniProt Gene Name
Ahsa1
UniProt Synonym Gene Names
AHA1
UniProt Entry Name
AHSA1_MOUSE
NCBI Protein Information
activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1

UniProt Comments for Ahsa1

AHSA1: Cochaperone that stimulates HSP90 ATPase activity. May affect a step in the endoplasmic reticulum to Golgi trafficking. Belongs to the AHA1 family.

Protein type: Chaperone

Cellular Component: endoplasmic reticulum; cytoplasm

Molecular Function: ATPase activator activity; chaperone binding

Biological Process: positive regulation of ATPase activity; response to stress
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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