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dmpO Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS804719

Background Info: Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.Scientific Background: The formation of free radicals and other highly reactive oxygen… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
DMPO, Monoclonal Antibody
Full Product Name
DMPO Antibody: Alkaline Phosphatase
Product Gene Name
anti-dmpO antibody
Product Synonym Names
5;5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide; DMPO nitrone adduct
Clonality
Monoclonal
Isotype
IgG1
Clone Number
N1664A
Host
Mouse
Species Reactivity
Species Independent
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
13,207 Da
Conjugate
Alkaline Phosphatase
Storage Buffer
TBS pH7.4, 50% glycerol, 0.05% sodium azide

Product Categories/Family for anti-dmpO antibody

Oxidative Stress
Applications Tested/Suitable for anti-dmpO antibody
Immuno-spin trapping, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC),Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-dmpO antibody
1-10ug/mL (ELISA),10ug/mL (ICC), 25ug (IP)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-dmpO antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.
Mouse Anti-dmpO Monoclonal Antibody – Cat# MBS804719

Western Blot (WB) of anti-dmpO antibody

Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.
Mouse Anti-dmpO Monoclonal Antibody, Western Blot (WB) – Cat# MBS804719

Product References and Citations for anti-dmpO antibody

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473- 1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73-75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427-436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251- 256. 7. Free Radic Biol Med. 2009 April 1; 46(7): 853-865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., Ehrenshaft, M., Bhattacharjee,S., Derterding, L.J., Bonini, M.G., Corbett, J., Kadiiska, M.B., Tomer K.B. and Mason, R.P. 2009 Free Radic. Med. and Biol. 46:454-461.

Other Notes

Small volumes of anti-dmpO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for dmpO purchase

MBS804719 is an antibody targeting dmpO. Also known as DMPO Antibody: Alkaline Phosphatase. Reactive in Species Independent. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for dmpO. It may not necessarily be applicable to this product.

NCBI GI #
51242284
NCBI Accession #
AAT99086.1
NCBI Official Full Name
DmpO
UniProt Protein Name
DmpO
UniProt Synonym Protein Names
DmpO
Protein Family
Phenol hydroxylase P4 protein
UniProt Gene Name
dmpO
UniProt Entry Name
Q68J58_PSEPU

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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