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MMP9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS804888

Background Info: Detects ~92kDa and ~82kDa (pro and active forms)Scientific Background: MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MMP9, Monoclonal Antibody
Full Product Name
MMP9 Antibody: ATTO 488
Product Gene Name
anti-Mmp9 antibody
Product Synonym Names
MMP-9; CLG4B; 82kDa matrix metalloproteinase-9; collagenease type 4 beta; GELB; Macrophage gelatinase; MANDP2; Type V collagenase; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; MMP9; Matrix metalloproteinase 9
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S51-82
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS804888)
ATTO 488 Conjugated Antibody: MMP9 Clone #S51-82 (MBS804888)
Molecular Weight
78,611 Da
3D Structure
ModBase 3D Structure for P50282
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Mmp9 antibody

Apoptosis; Neuroscience
Applications Tested/Suitable for anti-Mmp9 antibody
Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-Mmp9 antibody
1:1000 (WB)

Immunocytochemistry (ICC) of anti-Mmp9 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.
Mouse Anti-MMP9 Monoclonal Antibody – Cat# MBS804888

Western Blot (WB) of anti-Mmp9 antibody

Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).
Mouse Anti-MMP9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS804888

Product References and Citations for anti-Mmp9 antibody

1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391. 3. Sternlicht M.D., et al. (1999) Cell 98: 137-146. 4. Vu T.H., et al. (1998) Cell 93: 411-422. 5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.

Other Notes

Small volumes of anti-Mmp9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mmp9 purchase

MBS804888 is an antibody targeting Mmp9. Also known as MMP9 Antibody: ATTO 488. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Mmp9. It may not necessarily be applicable to this product.

NCBI GI #
13591993
NCBI GeneID
81687
NCBI Accession #
NP_112317.1
UniProt Primary Accession #
P50282
UniProt Related Accession #
P50282
GenBank Nucleotide #
NM_031055.1
NCBI Official Full Name
matrix metalloproteinase-9
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
Mmp9
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Synonym Protein Names
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Protein Family
Matrix metalloproteinase
UniProt Gene Name
Mmp9
UniProt Synonym Gene Names
MMP-9; GELB
UniProt Entry Name
MMP9_RAT
NCBI Protein Information
matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)

NCBI Summary for Mmp9

metalloproteinase involved in extracellular matrix remodeling and bone resorption [RGD, Feb 2006]

UniProt Comments for Mmp9

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments ().

Research Articles on Mmp9

1. Study indicates that NADPH oxidase subunit gp91phox contributes to induced expression of MMP-9 in ischemic brain tissue

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mmp9 antibody

 Reference Product  PubMed Publications
 Timp1 antibody  >286 publications with Mmp9 and Timp1
 Tnf antibody  >229 publications with Mmp9 and Tnf
 Vegfa antibody  >83 publications with Mmp9 and Vegfa
 Kitlg antibody  >80 publications with Mmp9 and Kitlg
 Ccl2 antibody  >62 publications with Mmp9 and Ccl2
 Cd44 antibody  >31 publications with Mmp9 and Cd44
 Fgf2 antibody  >29 publications with Mmp9 and Fgf2
 Il1b antibody  >24 publications with Mmp9 and Il1b
 Lcn2 antibody  >18 publications with Mmp9 and Lcn2

Pathways associated with anti-Mmp9 antibody

 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram

Diseases associated with anti-Mmp9 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >481 publications with Mmp9 and Inflammation
 Neoplasm Invasiveness Antibodies  >390 publications with Mmp9 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >343 publications with Mmp9 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >162 publications with Mmp9 and Breast Neoplasms
 Liver Diseases Antibodies  >146 publications with Mmp9 and Liver Diseases
 Aortic Diseases Antibodies  >97 publications with Mmp9 and Aortic Diseases
 Carcinoma, Squamous Cell Antibodies  >94 publications with Mmp9 and Carcinoma, Squamous Cell
 Aortic Aneurysm Antibodies  >91 publications with Mmp9 and Aortic Aneurysm
 Aortic Aneurysm, Abdominal Antibodies  >75 publications with Mmp9 and Aortic Aneurysm, Abdominal
 Carcinoma, Hepatocellular Antibodies  >70 publications with Mmp9 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-Mmp9 antibody

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >121 publications with Mmp9 and Connective Tissue
 Placenta Antibodies  >78 publications with Mmp9 and Placenta
 Uncharacterized Tissue Antibodies  >2 publications with Mmp9 and Uncharacterized Tissue
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