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SLC12A3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS805451

Function: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NCC (SLC12A3), Polyclonal Antibody
Full Product Name
NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal
Product Gene Name
Product Synonym Names
SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Dog
Purity/Purification
Affinity Purified
Concentration
1mg/mL (lot specific)
Specificity
Detects ~160kDa
Preparation and Storage
Store at -20°C; 1 year+ Avoid freeze/thaw cycles.
Shipping: Blue Ice or 4°C
Matching Pairs
Unconjugated Antibody: NCC (MBS805073)
ATTO 594 Conjugated Antibody: NCC (MBS805451)
Matching Pairs
Unconjugated Antibody: N/A (MBS805451)
ATTO 594 Conjugated Antibody: NCC (MBS805451)
Molecular Weight
~160kDa
3D Structure
ModBase 3D Structure for P55018
Conjugate
ATTO 594
Immunogen
Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal.
Certificate of Analysis
1ug/ml of MBS805451 was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-Rabbit IgG: HRP as the secondary antibody.
Research Areas
Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters.
Cellular Localization
Membrane
Sequence References
Gene ID:54300,6559
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-SLC12A3 antibody

Applications Tested/Suitable for anti-SLC12A3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), IEM
Application Notes for anti-SLC12A3 antibody
WB: 1:1000
Optimal dilutions for assays should be determined by the end user.

Immunohistochemistry (IHC) of anti-SLC12A3 antibody

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).
Rabbit Anti-SLC12A3 Polyclonal Antibody – Cat# MBS805451

Western Blot (WB) of anti-SLC12A3 antibody

Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.
Rabbit Anti-SLC12A3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS805451

Product References and Citations for anti-SLC12A3 antibody

1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

Other Notes

Small volumes of anti-SLC12A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC12A3 purchase

MBS805451 is an antibody targeting SLC12A3. Also known as NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal. Reactive in Human, Mouse, Rat, Dog. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC12A3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
solute carrier family 12 member 3 isoform 1
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/chloride transporter), member 3
NCBI Official Symbol
SLC12A3
NCBI Official Synonym Symbols
NCC; TSC; NCCT
UniProt Protein Name
Solute carrier family 12 member 3
UniProt Synonym Protein Names
Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
Protein Family
UniProt Gene Name
Slc12a3
UniProt Synonym Gene Names
Tsc
UniProt Entry Name
S12A3_RAT
NCBI Protein Information
solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter

NCBI Summary for SLC12A3

This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for SLC12A3

NCCT: Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Cellular Component: apical plasma membrane; plasma membrane; integral to membrane; cytosol; vesicle

Molecular Function: sodium ion transmembrane transporter activity; transporter activity; sodium:chloride symporter activity; cation:chloride symporter activity

Biological Process: transport; sodium ion transport; chloride transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-SLC12A3 antibody

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