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Complement Component 5a Receptor 1 (C5aR1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS080546

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of C5AR1 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Complement Component 5a Receptor 1 (C5aR1), ELISA Kit
Full Product Name
Rat Complement Component 5a Receptor 1 ELISA Kit
Product Gene Name
C5aR1 elisa kit
Species Reactivity
Rat
Assay Type
Sandwich (Quantitative)
Detection Range
6.25 ng/ml - 200 ng/ml.
Sensitivity
1.0 ng/ml.
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
39,336 Da
OMIM
113995
3D Structure
ModBase 3D Structure for P21730

Other Notes

Small volumes of C5aR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C5aR1 purchase

MBS080546 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 5a Receptor 1 (C5aR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C5aR1. The ELISA analytical biochemical technique of the MBS080546 kit is based on C5aR1 antibody-C5aR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5aR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5aR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for C5aR1. It may not necessarily be applicable to this product.

NCBI GI #
4502509
NCBI GeneID
728
NCBI Accession #
NP_001727.1
UniProt Primary Accession #
P21730
UniProt Related Accession #
P21730
GenBank Nucleotide #
NM_001736.3
NCBI Official Full Name
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Synonym Full Names
complement component 5a receptor 1
NCBI Official Symbol
C5AR1
NCBI Official Synonym Symbols
C5A; C5AR; C5R1; CD88
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor 1
UniProt Synonym Protein Names
C5a anaphylatoxin chemotactic receptor
Protein Family
C5a anaphylatoxin chemotactic receptor
UniProt Gene Name
C5AR1
UniProt Synonym Gene Names
C5AR; C5R1; C5a-R; C5aR
UniProt Entry Name
C5AR1_HUMAN
NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1

UniProt Comments for C5aR1

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; apical part of cell; integral to plasma membrane; basolateral plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; apoptosis; activation of MAPK activity; response to lipopolysaccharide; chemotaxis; cell proliferation in hindbrain; signal transduction; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation
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Research Articles on C5aR1

1. High levels of CD88 are associated with poor prognosis of NSCLC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with C5aR1 elisa kit

 Reference Product  PubMed Publications
 C3AR1 elisa kit  >2 publications with C5aR1 and C3AR1
 GNAI2 elisa kit  >1 publications with C5aR1 and GNAI2
 FPR1 elisa kit  >1 publications with C5aR1 and FPR1

Pathways associated with C5aR1 elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram

Diseases associated with C5aR1 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with C5aR1 and Inflammation
 Immune System Diseases Antibodies  >19 publications with C5aR1 and Immune System Diseases
 Cardiovascular Diseases Antibodies  >11 publications with C5aR1 and Cardiovascular Diseases
 Neoplasms Antibodies  >6 publications with C5aR1 and Neoplasms
 Necrosis Antibodies  >6 publications with C5aR1 and Necrosis
 Kidney Diseases Antibodies  >4 publications with C5aR1 and Kidney Diseases
 Nervous System Diseases Antibodies  >4 publications with C5aR1 and Nervous System Diseases
 Fibrosis Antibodies  >3 publications with C5aR1 and Fibrosis
 Eye Diseases Antibodies  >3 publications with C5aR1 and Eye Diseases
 Lung Injury Antibodies  >3 publications with C5aR1 and Lung Injury

Organs/Tissues associated with C5aR1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >48 publications with C5aR1 and Blood
 Bone Marrow Antibodies  >4 publications with C5aR1 and Bone Marrow
 Intestine Antibodies  >4 publications with C5aR1 and Intestine
 Brain Antibodies  >4 publications with C5aR1 and Brain
 Placenta Antibodies  >3 publications with C5aR1 and Placenta
 Uterus Antibodies  >1 publications with C5aR1 and Uterus
 Prostate Antibodies  >1 publications with C5aR1 and Prostate
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