RPS6KB2 Antibody – Rabbit Polyclonal

Background Info: Detects a ~70kDa protein corresponding to the molecular mass of p70 S6K on SDS PAGE immunoblots.

Scientific Background: Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.

1. Gout, I. et al. (1998). J Biol Chem. 273 (46):30061-30064. 2. Val'Ovka, TI. et al. (2000). Ukr Biokhim Zh. 72(3):31-7. 3. Kawasome, H. et al. (1998) Proc. Natl. Acad. Sci. 95 4. Montagne, J. et al. 1999) Science 285 5. Shima, H. et al. (1998) EMBO J 17: 6649- 6659. 6. Lee-Fruman, K. et al. (1999) Oncogene 18: 5108- 5114.

Small volumes of anti-RPS6KB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS805618 is an antibody targeting RPS6KB2. Also known as p70 S6K Antibody: PerCP. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit.