• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SLC12A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS805636

Background Info: Detects ~160kDaScientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the… More details

$585 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NKCC2 (Slc12a1), Polyclonal Antibody
Full Product Name
NKCC2 Antibody: PerCP
Product Gene Name
anti-Slc12a1 antibody
Product Synonym Names
Slc12a1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human (weak), Mouse, Rat
Form/Format
Affinity Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Matching Pairs
Unconjugated Antibody: NKCC2 (MBS805560)
PerCP Conjugated Antibody: NKCC2 (MBS805636)
Matching Pairs
Unconjugated Antibody: N/A (MBS805636)
PerCP Conjugated Antibody: NKCC2 (MBS805636)
Molecular Weight
120,417 Da
3D Structure
ModBase 3D Structure for Q5PQV1
Conjugate
PerCP
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide.

Product Categories/Family for anti-Slc12a1 antibody

Ion Channels; Transporters
Applications Tested/Suitable for anti-Slc12a1 antibody
Western Blot (WB), Immunoblot (IB)
Application Notes for anti-Slc12a1 antibody
1:1000 (WB)

Western Blot (WB) of anti-Slc12a1 antibody

Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.
Rabbit Anti-SLC12A1 Polyclonal Antibody – Cat# MBS805636

Product References and Citations for anti-Slc12a1 antibody

1.Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2.Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.

Other Notes

Small volumes of anti-Slc12a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Slc12a1 purchase

MBS805636 is an antibody targeting Slc12a1. Also known as NKCC2 Antibody: PerCP. Reactive in Human (weak), Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Slc12a1. It may not necessarily be applicable to this product.

NCBI GI #
162287159
NCBI GeneID
25065
NCBI Accession #
NP_062007
UniProt Primary Accession #
Q5PQV1
UniProt Related Accession #
P55016
GenBank Nucleotide #
NM_019134.2
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/potassium/chloride transporter), member 1
NCBI Official Symbol
Slc12a1
NCBI Official Synonym Symbols
BSC1; Nkcc2
UniProt Protein Name
RCG26347, isoform CRA_c
UniProt Synonym Protein Names
RCG26347, isoform CRA_c; Slc12a1 protein; Solute carrier family 12 member 1
Protein Family
Solute carrier family
UniProt Gene Name
Slc12a1
UniProt Synonym Gene Names
rCG_26347
UniProt Entry Name
Q5PQV1_RAT
NCBI Protein Information
solute carrier family 12 member 1

NCBI Summary for Slc12a1

has bumetamide-sensitive Na(+)-(K+)-Cl- symporter activity; may play a role in sodium and chloride transport in the kidney [RGD, Feb 2006]

UniProt Comments for Slc12a1

NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Cellular Component: cell surface; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: sodium:potassium:chloride symporter activity; cation:chloride symporter activity

Biological Process: diterpenoid metabolic process; sodium ion transport; protein insertion into membrane from inner side; chloride transport; excretion; kidney development; potassium ion transport; chemical homeostasis
Show more

Research Articles on Slc12a1

1. Report tubuloglomerular and connecting tubuloglomerular feedback during inhibition of various Na transporters in the nephron.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Slc12a1 antibody

 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-Slc12a1 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >73 publications with Slc12a1 and Kidney Diseases
 Hypertension Antibodies  >72 publications with Slc12a1 and Hypertension
 Bartter Syndrome Antibodies  >39 publications with Slc12a1 and Bartter Syndrome
 Disease Models, Animal Antibodies  >27 publications with Slc12a1 and Disease Models, Animal
 Hypokalemia Antibodies  >24 publications with Slc12a1 and Hypokalemia
 Polyuria Antibodies  >18 publications with Slc12a1 and Polyuria
 Nervous System Diseases Antibodies  >13 publications with Slc12a1 and Nervous System Diseases
 Renal Insufficiency Antibodies  >10 publications with Slc12a1 and Renal Insufficiency
 Hypotension Antibodies  >7 publications with Slc12a1 and Hypotension
 Heart Diseases Antibodies  >7 publications with Slc12a1 and Heart Diseases

Organs/Tissues associated with anti-Slc12a1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >225 publications with Slc12a1 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000