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KRAS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS805817

Background Info: Detects ~21kDa.Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Ras (Kras), Polyclonal Antibody
Full Product Name
RAS Antibody: ATTO 390
Product Gene Name
Product Synonym Names
GTPase Hras; GTPase Kras; GTPase Nras; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
3,693 Da
3D Structure
ModBase 3D Structure for B2KGV5
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Kras antibody

Applications Tested/Suitable for anti-Kras antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Kras antibody
1:1000 (WB)

Immunohistochemistry (IHC) of anti-Kras antibody

Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis.
Rabbit Anti-KRAS Polyclonal Antibody – Cat# MBS805817

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Kras antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction.
Rabbit Anti-KRAS Polyclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS805817

Product References and Citations for anti-Kras antibody

1. Boguski M.S. and McCormick F. (1993) Nature 366: 643-654. 2. Avruch J., et al., (1994) Trends Biochem. Sci.19: 279-283. 3. Buday L. and Downward J. (1993) Cell 73: 611-620. 4. Huang D.C., et al., (1993) Mol. Cell Biol. 13: 2420-2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682-4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114- 131.

Other Notes

Small volumes of anti-Kras antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Kras purchase

MBS805817 is an antibody targeting Kras. Also known as RAS Antibody: ATTO 390. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Kras. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
GTPase NRas
UniProt Protein Name
GTPase KRas
UniProt Synonym Protein Names
GTPase KRas
Protein Family
UniProt Gene Name
Kras
UniProt Entry Name
B2KGV5_MOUSE

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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