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Bassoon (BSN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612017

Function: Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles… More details

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Product Overview

Online Datasheet in PDF
Product Name
Bassoon (BSN), Polyclonal Antibody
Full Product Name
Bassoon Antibody
Product Gene Name
anti-BSN antibody
Product Synonym Gene Name
KIAA0434, ZNF231
Product Synonym Names
Bassoon protein; bsn; BSN_HUMAN; KIAA0434; Neuronal double zinc finger protein; Presynaptic cytomatrix protein; Protein bassoon; Zinc finger protein 231; ZNF 231; ZNF231
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Xenopus(86%)
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
1mg/ml (lot specific)
Specificity
Bassoon Antibody detects endogenous levels of total Bassoon.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Bassoon peptide (MBS9621961) is used for blocking the activity of Bassoon antibody (MBS9612017)
Molecular Weight
Observed Molecular Weight: (Observed)416kD.
Predicted Molecular Weight: (Calculated)416kDa.
OMIM
604020
3D Structure
ModBase 3D Structure for Q9UPA5
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Bassoon, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-BSN antibody
ELISA (EIA)
Application Notes for anti-BSN antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000

Western Blot (WB) of anti-BSN antibody

Western blot analysis of extracts from Rat brain, using Bassoon Antibody. The lane on the left was treated with blocking peptide.
Rabbit Anti-BSN Polyclonal Antibody – Cat# MBS9612017

Immunohistochemistry (IHC)-Paraffin of anti-BSN antibody

MBS9612017 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-BSN Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612017

Immunohistochemistry (IHC)-Paraffin of anti-BSN antibody

MBS9612017 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-BSN Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612017

Other Notes

Small volumes of anti-BSN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BSN purchase

MBS9612017 is an antibody targeting BSN. Also known as Bassoon Antibody. Reactive in Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Xenopus(86%). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BSN. It may not necessarily be applicable to this product.

NCBI GI #
229463040
NCBI GeneID
8927
UniProt Primary Accession #
Q9UPA5
UniProt Related Accession #
Q9UPA5
NCBI Official Full Name
Protein bassoon
NCBI Official Synonym Full Names
bassoon presynaptic cytomatrix protein
NCBI Official Symbol
BSN
NCBI Official Synonym Symbols
ZNF231
UniProt Protein Name
Protein bassoon
UniProt Synonym Protein Names
Zinc finger protein 231
Protein Family
Extracellular ribonuclease
UniProt Gene Name
BSN
UniProt Synonym Gene Names
KIAA0434; ZNF231
UniProt Entry Name
BSN_HUMAN
NCBI Protein Information
protein bassoon

NCBI Summary for BSN

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008]
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UniProt Comments for BSN

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: axon; postsynaptic density; dendrite; presynaptic active zone; excitatory synapse; cell junction; nucleus

Molecular Function: metal ion binding

Biological Process: synaptic transmission
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Research Articles on BSN

1. Three missense mutations in BSN that are very rarely listed in databases of healthy subjects were found in four sporadic progressive supranuclear palsy cases. Western blot analysis of tau following the overexpression of wild-type or mutated BSN revealed the possibility that wild-type BSN reduced tau accumulation, while mutated BSN lost this function.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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