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SOD (Ec) (SOD3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806035

Background Info: Identifies a band ~35kD on WBScientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2"… More details

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Product Overview

Product Name
SOD (EC) (SOD3), Polyclonal Antibody
Full Product Name
SOD (EC) Antibody: ATTO 594
Product Gene Name
anti-SOD3 antibody
Product Synonym Names
Extra-cellular superoxide dismutase; SOD3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Peptide Affinity Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
25,851 Da
OMIM
185490
3D Structure
ModBase 3D Structure for P08294
Conjugate
ATTO 594
Storage Buffer
PBS pH 7.4; 50% glycerol, 0.09% azide

Product Categories/Family for anti-SOD3 antibody

Oxidative Stress
Applications Tested/Suitable for anti-SOD3 antibody
Western Blot (WB)
Application Notes for anti-SOD3 antibody
1:1000 for WB

Immunocytochemistry (ICC) of anti-SOD3 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.
Rabbit Anti-SOD3 Polyclonal Antibody – Cat# MBS806035

Immunocytochemistry (ICC) of anti-SOD3 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.
Rabbit Anti-SOD3 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS806035

Western Blot (WB) of anti-SOD3 antibody

Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~35 kDa SOD (EC) protein using Rabbit Anti-SOD (EC) Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35 kDa. Other Band(s): 50 kDa.
Rabbit Anti-SOD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS806035

Product References and Citations for anti-SOD3 antibody

1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O'Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479-3491

Other Notes

Small volumes of anti-SOD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOD3 purchase

MBS806035 is an antibody targeting SOD3. Also known as SOD (EC) Antibody: ATTO 594. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOD3. It may not necessarily be applicable to this product.

NCBI GI #
118582275
NCBI GeneID
6649
NCBI Accession #
NP_003093.2
UniProt Primary Accession #
P08294
UniProt Secondary Accession #
Q5U781; Q6FHA2
UniProt Related Accession #
P08294
GenBank Nucleotide #
NM_003102.2
NCBI Official Full Name
extracellular superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 3, extracellular
NCBI Official Symbol
SOD3
NCBI Official Synonym Symbols
EC-SOD
UniProt Protein Name
Extracellular superoxide dismutase [Cu-Zn]
Protein Family
Extracellular superoxide dismutase
UniProt Gene Name
SOD3
UniProt Synonym Gene Names
EC-SOD
UniProt Entry Name
SODE_HUMAN
NCBI Protein Information
extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]

NCBI Summary for SOD3

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOD3

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Secreted; Secreted, signal peptide; EC 1.15.1.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia
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Research Articles on SOD3

1. Loss of SOD3 is associated with prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SOD3 antibody

 Reference Product  PubMed Publications
 SOD2 antibody  >85 publications with SOD3 and SOD2
 CAT antibody  >26 publications with SOD3 and CAT
 ATP7A antibody  >5 publications with SOD3 and ATP7A
 ATOX1 antibody  >4 publications with SOD3 and ATOX1
 ACE antibody  >1 publications with SOD3 and ACE
 FBLN5 antibody  >1 publications with SOD3 and FBLN5

Pathways associated with anti-SOD3 antibody

 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Vitamin B12 Metabolism Pathway antibodies  Vitamin B12 Metabolism Pathway Diagram
 Superoxide Radicals Degradation Pathway antibodies  Superoxide Radicals Degradation Pathway Diagram

Diseases associated with anti-SOD3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >70 publications with SOD3 and Cardiovascular Diseases
 Lung Diseases Antibodies  >50 publications with SOD3 and Lung Diseases
 Inflammation Antibodies  >47 publications with SOD3 and Inflammation
 Disease Models, Animal Antibodies  >41 publications with SOD3 and Disease Models, Animal
 Hypertension Antibodies  >33 publications with SOD3 and Hypertension
 Heart Diseases Antibodies  >31 publications with SOD3 and Heart Diseases
 Lung Injury Antibodies  >22 publications with SOD3 and Lung Injury
 Pulmonary Disease, Chronic Obstructive Antibodies  >19 publications with SOD3 and Pulmonary Disease, Chronic Obstructive
 Nervous System Diseases Antibodies  >19 publications with SOD3 and Nervous System Diseases
 Fibrosis Antibodies  >16 publications with SOD3 and Fibrosis

Organs/Tissues associated with anti-SOD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >85 publications with SOD3 and Vascular
 Lung Antibodies  >71 publications with SOD3 and Lung
 Muscle Antibodies  >47 publications with SOD3 and Muscle
 Heart Antibodies  >44 publications with SOD3 and Heart
 Kidney Antibodies  >25 publications with SOD3 and Kidney
 Brain Antibodies  >22 publications with SOD3 and Brain
 Bone Antibodies  >11 publications with SOD3 and Bone
 Skin Antibodies  >11 publications with SOD3 and Skin
 Eye Antibodies  >9 publications with SOD3 and Eye
 Prostate Antibodies  >9 publications with SOD3 and Prostate
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