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NRAS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806299

Background Info: Detects ~21kDa.Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors… More details

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Product Overview

Product Name
Ras (Nras), Polyclonal Antibody
Full Product Name
RAS Antibody: RPE
Product Gene Name
anti-Nras antibody
Product Synonym Names
GTPase Hras; GTPase Kras; GTPase Nras; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
21,199 Da
3D Structure
ModBase 3D Structure for B2KGV5
Conjugate
RPE
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Nras antibody

Cell Signaling
Applications Tested/Suitable for anti-Nras antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Nras antibody
1:1000 (WB)

Immunohistochemistry (IHC) of anti-Nras antibody

Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis.
Rabbit Anti-NRAS Polyclonal Antibody – Cat# MBS806299

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Nras antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction.
Rabbit Anti-NRAS Polyclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS806299

Product References and Citations for anti-Nras antibody

1. Boguski M.S. and McCormick F. (1993) Nature 366: 643-654. 2. Avruch J., et al., (1994) Trends Biochem. Sci.19: 279-283. 3. Buday L. and Downward J. (1993) Cell 73: 611-620. 4. Huang D.C., et al., (1993) Mol. Cell Biol. 13: 2420-2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682-4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114- 131.

Other Notes

Small volumes of anti-Nras antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nras purchase

MBS806299 is an antibody targeting Nras. Also known as RAS Antibody: RPE. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nras. It may not necessarily be applicable to this product.

NCBI GI #
111154109
NCBI GeneID
18176
NCBI Accession #
NP_035067.2
UniProt Primary Accession #
B2KGV5
UniProt Related Accession #
P08556
GenBank Nucleotide #
NM_010937.2
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
neuroblastoma ras oncogene
NCBI Official Symbol
Nras
NCBI Official Synonym Symbols
N-ras; AV095280
UniProt Protein Name
GTPase NRas
UniProt Synonym Protein Names
Transforming protein N-Ras
Protein Family
GTPase
UniProt Gene Name
Nras
UniProt Entry Name
RASN_MOUSE
NCBI Protein Information
GTPase NRas; transforming protein N-Ras

UniProt Comments for Nras

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Ras; Oncoprotein; G protein, monomeric; G protein

Cellular Component: Golgi apparatus; membrane; plasma membrane; intracellular

Molecular Function: GTPase activity; GTP binding; protein complex binding; nucleotide binding

Biological Process: negative regulation of skeletal muscle development; regulation of cell cycle; small GTPase mediated signal transduction; Ras protein signal transduction; signal transduction
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Research Articles on Nras

1. These observations provide genetic evidence for an essential role of Ras proteins in the control of keratinocyte and epidermal proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Nras antibody

 Reference Product  PubMed Publications
 Egfr antibody  >127 publications with Nras and Egfr
 Jun antibody  >116 publications with Nras and Jun
 Akt1 antibody  >43 publications with Nras and Akt1
 Ccnd1 antibody  >29 publications with Nras and Ccnd1
 Src antibody  >9 publications with Nras and Src
 Igf1 antibody  >1 publications with Nras and Igf1

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