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TRAP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806361

Background Info: Detects TRAP1. Detects ~75 kDa, multiple isoforms.Scientific Background: The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is… More details

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Product Overview

Product Name
TRAP1, Polyclonal Antibody
Full Product Name
TRAP1 Antibody: Biotin
Product Gene Name
Product Synonym Names
Heat shock protein 75; Heat shock protein 75 kDa; Heat shock protein 75 kDa mitochondrial; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
Protein A Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
74,267 Da
OMIM
606219
3D Structure
ModBase 3D Structure for Q12931
Conjugate
Biotin
Storage Buffer
PBS pH 7.4, 0.09% azide, 50% glycerol.

Product Categories/Family for anti-TRAP1 antibody

Applications Tested/Suitable for anti-TRAP1 antibody
Western Blot (WB)
Application Notes for anti-TRAP1 antibody
1:1000 (WB)

Immunocytochemistry (ICC) of anti-TRAP1 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.
Rabbit Anti-TRAP1 Polyclonal Antibody – Cat# MBS806361

Western Blot (WB) of anti-TRAP1 antibody

Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~75 kDa TRAP1 protein using Rabbit Anti-TRAP1 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 12 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa.
Rabbit Anti-TRAP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS806361

Immunocytochemistry (ICC) of anti-TRAP1 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.
Rabbit Anti-TRAP1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS806361

Product References and Citations for anti-TRAP1 antibody

1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.

Other Notes

Small volumes of anti-TRAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRAP1 purchase

MBS806361 is an antibody targeting TRAP1. Also known as TRAP1 Antibody: Biotin. Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 75 kDa, mitochondrial isoform 1
NCBI Official Synonym Full Names
TNF receptor-associated protein 1
NCBI Official Symbol
TRAP1
NCBI Official Synonym Symbols
HSP75; HSP 75; HSP90L; TRAP-1
UniProt Protein Name
Heat shock protein 75 kDa, mitochondrial
UniProt Synonym Protein Names
TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1
Protein Family
UniProt Gene Name
TRAP1
UniProt Synonym Gene Names
HSP75; HSP 75; TRAP-1
UniProt Entry Name
TRAP1_HUMAN
NCBI Protein Information
heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein

NCBI Summary for TRAP1

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
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UniProt Comments for TRAP1

HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; membrane; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space; lipid particle

Molecular Function: protein binding; unfolded protein binding; tumor necrosis factor receptor binding; protein kinase binding; ATP binding

Biological Process: response to stress
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRAP1 antibody

 Reference Product  PubMed Publications
 PINK1 antibody  >9 publications with TRAP1 and PINK1

Pathways associated with anti-TRAP1 antibody

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