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Piccolo (PCLO) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806832

Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Piccolo (PCLO), Polyclonal Antibody
Full Product Name
Piccolo Antibody
Product Gene Name
Product Synonym Names
ACZ; Aczonin; Brain-derived HLMN protein; DKFZp779G1236; KIAA0559; Multidomain presynaptic cytomatrix protein; PCLO; PCLO_HUMAN; Piccolo (presynaptic cytomatrix protein); Piccolo mouse homolog of Pico; Presynaptic cytomatrix protein; Protein piccolo
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
47.35mM Phosphate, 2.15mM MES, 1.033mM EDTA
Purity/Purification
Protein A purified
Concentration
1mg/mL (lot specific)
Specificity
Detects ~550kDa corresponding to the molecular weight of Piccolo. Multiple isoforms can be detected. No cross-reactivity to Bassoon.
Preparation and Storage
Store at 4°C ; >1 year . Avoid freeze / thaw cycles.
Matching Pairs
Unconjugated Antibody: Piccolo (MBS806832)
ATTO 390 Conjugated Antibody: Piccolo (MBS805606)
Matching Pairs
Unconjugated Antibody: Piccolo (MBS806832)
Biotin Conjugated Antibody: Piccolo (MBS805748)
OMIM
604918
3D Structure
ModBase 3D Structure for Q9Y6VO
Certificate of Analysis
1ug/mL of MBS806832 was sufficient for detection of Piccolo in 10ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Research Areas
Cell Markers, Neuron Markers, Neuroscience, Presynaptic Markers
Cellular Localization
Cell Junction, Synapse
Immunogen
Recombinant Full length Protein, BC001304.1

Product Categories/Family for anti-PCLO antibody

Applications Tested/Suitable for anti-PCLO antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PCLO antibody
WB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.

Immunohistochemistry (IHC) of anti-PCLO antibody

Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.
Rabbit Anti-PCLO Polyclonal Antibody – Cat# MBS806832

Western Blot (WB) of anti-PCLO antibody

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000.
Rabbit Anti-PCLO Polyclonal Antibody, Western Blot (WB) – Cat# MBS806832

Product References and Citations for anti-PCLO antibody

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

Other Notes

Small volumes of anti-PCLO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCLO purchase

MBS806832 is an antibody targeting PCLO. Also known as Piccolo Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCLO. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein piccolo isoform 2
NCBI Official Synonym Full Names
piccolo presynaptic cytomatrix protein
NCBI Official Symbol
PCLO
NCBI Official Synonym Symbols
ACZ
UniProt Protein Name
Protein piccolo
UniProt Synonym Protein Names
Aczonin
Protein Family
UniProt Gene Name
PCLO
UniProt Synonym Gene Names
ACZ; KIAA0559
UniProt Entry Name
PCLO_HUMAN
NCBI Protein Information
protein piccolo; aczonin; piccolo (presynaptic cytomatrix protein)

NCBI Summary for PCLO

The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for PCLO

piccolo: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: synaptic vesicle; cytoskeleton; membrane; postsynaptic density; synapse; cell junction

Molecular Function: calcium-dependent phospholipid binding; transporter activity; calcium ion binding; profilin binding

Biological Process: regulation of exocytosis; synaptic vesicle exocytosis; cAMP-mediated signaling; insulin secretion; cytoskeleton organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 3
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PCLO antibody

 Products by Pathway  Pathway Diagram
 Insulin Secretion Pathway antibodies  Insulin Secretion Pathway Diagram
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