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AHA1 Yeast Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806931

Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Aha1, Recombinant Protein
Full Product Name
Recombinant Yeast AHA1
Product Gene Name
Product Synonym Names
Aha1; SHSA1; HspC322; p38
Host
Yeast
Species Reactivity
Yeast
Form/Format
Affinity Purified
Purity/Purification
>90%
Concentration
1.8mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
~40.5kDa
3D Structure
ModBase 3D Structure for Q12449
Certificate of Analysis
This product has been certified >90% pure using SDS - PAGE analysis.
Conjugate Tag
His tag
Expression System
E Coli
Storage Buffer
25mM Tris buffer pH7.2, 150mM NaCl, 0.1mM DTT, 50% glycerol

Product Categories/Family for AHA1 recombinant protein

Applications Tested/Suitable for AHA1 recombinant protein
Western Blot (WB) control, functional/activity studies

Testing Data of AHA1 recombinant protein

SDS PAGE.
Yeast AHA1 Recombinant Protein – Cat# MBS806931

Testing Data #2 of AHA1 recombinant protein

ATPase assay using yeast Hsp82 activated by. Reaction conditions are 25mM Hepes pH7.2, 2.2mM NaCl, 5mM MgCl2, 2mM ATP, 1mM PEP, 1mM DTT, PK/LDH, 300uM NADH
Yeast AHA1 Recombinant Protein, Testing Data #2 – Cat# MBS806931

Product References and Citations for AHA1 recombinant protein

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

Other Notes

Small volumes of AHA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AHA1 purchase

MBS806931 is a recombinant protein targeting AHA1. Also known as Recombinant Yeast AHA1. Reactive in Yeast. Host: Yeast.

Additional Details

NCBI/Uniprot data below describe general gene information for AHA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Aha1p
NCBI Official Symbol
AHA1
UniProt Protein Name
Hsp90 co-chaperone AHA1
UniProt Synonym Protein Names
Activator of Hsp90 ATPase protein 1
Protein Family
UniProt Gene Name
AHA1
UniProt Synonym Gene Names
YDR214W; YD8142.16; YD8142B.06
UniProt Entry Name
AHA1_YEAST
NCBI Protein Information
Aha1p

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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