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Receptor Alpha4 (GABA-A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS807009

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GABA-A Receptor Alpha4, Monoclonal Antibody
Full Product Name
Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1
Product Gene Name
Product Synonym Names
GABA A receptor subunit alpha 4 Antibody; GABRA4 Antibody; Gamma aminobutyric acid A receptor Alpha 4 Antibody; GABA-A Receptor Alpha4 Antibody: FITC; Mouse Anti-Rat GABA A Receptor Monoclonal, Clone S398A-34; Anti-GABA-A Receptor Alpha4; GABA-A Receptor Alpha4 Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S398A-34
Host
Mouse
Species Reactivity
Mouse, Rat
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C
Matching Pairs
Unconjugated Antibody: N/A (MBS807009)
FITC Conjugated Antibody: GABA-A Receptor Alpha4 Clone #S398A-34 (MBS807009)
Molecular Weight
Detects ~60kDa.
3D Structure
ModBase 3D Structure for P28471
Immunogen
Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Immunogen Species
Rat
COA
A 1:100 dilution was sufficient for detection of GABA-A R, Alpha4 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Conjugate
FITC
Cellular Localization
Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane
Applications Tested/Suitable for anti-GABA-A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GABA-A antibody
Western Blot: 1:1000

Western Blot (WB) of anti-GABA-A antibody

Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.
Mouse Anti-GABA-A Monoclonal Antibody – Cat# MBS807009

Product References and Citations for anti-GABA-A antibody

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Grabenstatter H.L., et al. (2014) Epilepsia. 55(11): 1826-1833. 4. Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.

Other Notes

Small volumes of anti-GABA-A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GABA-A purchase

MBS807009 is an antibody targeting GABA-A. Also known as Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1. Reactive in Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GABA-A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit alpha-4
NCBI Official Synonym Full Names
gamma-aminobutyric acid type A receptor alpha4 subunit
NCBI Official Symbol
Gabra4
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit alpha-4
UniProt Synonym Protein Names
GABA(A) receptor subunit alpha-4
UniProt Gene Name
Gabra4
UniProt Entry Name
GBRA4_RAT
NCBI Protein Information
gamma-aminobutyric acid receptor subunit alpha-4

NCBI Summary for GABA-A

alpha 4 subunit of the GABA-A receptor which binds gamma-aminobutyric acid; involved in mood and anxiety [RGD, Feb 2006]

UniProt Comments for GABA-A

GABRA4: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily.

Protein type: Channel, ligand-gated; Transporter; Transporter, ion channel; Channel, chloride; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cell junction; postsynaptic membrane

Molecular Function: chloride channel activity; extracellular ligand-gated ion channel activity; GABA-A receptor activity

Biological Process: central nervous system development; gamma-aminobutyric acid signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-GABA-A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >67 publications with GABA-A and Brain
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